摘要
目的：观察先心病病儿在围体外循环（ＣＰＢ）期红细胞膜表面补体Ｃ３ｂ受体（ＲＢＣＣＲ１）和膜流动性的变化。方法：随机选择２０例病儿在７个不同时点经中心静脉取血，ＥＬＩＳＡ法测定ＲＢＣＣＲ１表达量，荧光偏振法测ＲＢＣ膜流动性，生化法测全血ＧＳＨＰｘ和ＲＢＣＧＳＨ含量。结果：受检期间ＲＢＣＣＲ１表达量和膜流动性均显著下降，术后１６８小时未恢复术前水平，二者呈显著正相关（Ｐ＜０．００１）。开放升主动脉至术后２４小时，全血ＧＳＨＰｘ活力显著下降，与ＲＢＣＣＲ１表达量呈显著正相关（Ｐ＜０．００１），与微粘度（η）呈负相关（Ｐ＜０．０５）。结论：围ＣＰＢ期的创伤显示ＲＢＣＣＲ１表达量和膜流动性均下降且恢复缓慢，这对ＲＢＣ的免疫功能有较大影响。
Aim:To study the changes and clinical significance of RBC CR 1 and RBC membrane fluidity during cardiopulmonary bypass(CPB).Clinical material and method:The expression of RBC CR 1 was studied in 20 patients using ELISA method during open heart surgery under CPB.The RBC membrane fluidity was determined by fluorescence polarization technique.Blood samples were taken at seven different time intervals.The activity of GSH Px and RBC GSH level were measured simultaneously.Result:The expression of RBC CR 1 and RBC membrane fluidity were significantly decreased.There were a positive correlation between RBC CR 1 and membrane microviscosity.The activity of GSH Px was markedly decreased and it hnd positive correlation with RBC CR 1 and negative correlation with microviscosity.Conclusion:The changes of RBC CR 1 and RBC membrane fluidity is closely related to cardiopulmonary bypass.It may be implicative in some postoperative complications.
出处
《中华胸心血管外科杂志》
CSCD
北大核心
1999年第1期7-9,共3页
Chinese Journal of Thoracic and Cardiovascular Surgery
关键词
体外循环
红细胞免疫功能
围手术期
RBC CR 1 RBC membrane fluidity Cardiopulmonary bypass
