摘要
目的观察抗胆碱药盐酸苯环壬酯(PH)和丁溴东莨菪碱(SB)对大鼠海马齿状回诱发电位及长时程增强现象(LTP)的影响。方法采用电刺激成年大鼠内侧穿行通路(MPP),记录海马齿状回(DG)颗粒细胞诱发电位的方法,观察PH(0.2-17.4mg/kg,ip)对以诱发电位的群峰电位(PS)幅度和高频刺激诱导PS幅度LTP现象的影响。并与SB(0.3-20mg/kg,ip)进行比较。结果 PH对MPP-DG通路诱发电位PS幅度有促进作用,对高频诱导的LTP现象没有影响。SB对MPP-DG通路诱发电位PS幅度和高频诱导的LTP现象均没有影响。结论 PH对麻醉大鼠海马颗粒细胞有兴奋作用;SB对大鼠海马颗粒细胞的兴奋性没有影响。
Objective To study the effect of the anti-cholinergic drug Phencynonate and scopolamine on extracellular field evoked potentials and its LTP in the dentate gyrus of hippocampus in urethane-anaesthetized rats.Methods Single pathway recording of evoked field potentials was made from the dentate granule cells of hippocampal hemisphere in response to stimulation of the ipsilateral medial perforant path（MPP）.The amplitude of population spike（PS amplitude）,was employed to evaluate the effects of drug on the overall changes in cellular responses.Results Phencynonate hydrochloride（0.2-17.4 mg/kg,i.p.） could potentiate basal synaptic transportation by increasing the PS amplitude.Scopolamine butyl bromide（0.3-20 mg/kg,i.p.） could not affect the basal PS amplitude.Phencynonate hydrochloride and scopolamine butyl bromide did not alter either the PS amplitude LTP by conditioned tetanus（60Hz,30 pulses）.Conclusion Anti-cholinergic drug Phencynonate can potentiate the excitability of the cells in dentate gyrus of hippocampus,while scopolamine could not potentiate the excitability in the same test situation.
出处
《宁夏医学杂志》
CAS
2010年第7期592-594,I0001,共4页
Ningxia Medical Journal
