摘要
目的探讨胃粘膜相关淋巴组织（ＭＡＬＴ）淋巴瘤与幽门螺杆菌（Ｈｐ）感染的关系和各种不同层次检测方法的诊断价值。方法对３１例ＭＡＬＴ淋巴瘤作Ｇｉｅｍｓａ染色查Ｈｐ、免疫组化染色、半巢式ＰＣＲ扩增进行ＩｇＨ基因重排，检测其单克隆性。以同期胃淋巴细胞性胃炎（ＬＧ）、无关疾病胃粘膜作对照。结果胃ＭＡＬＴ淋巴瘤中Ｈｐ感染率８３．９％，单克隆性重排８７．１％，远高于对照组。但免疫组化染色显示的轻链限制性仅５５．６％。结论ＰＣＲ法检测ＩｇＨ基因重排优于免疫组化染色检测轻链限制性。在ＬＧ中发现２６．９％有单克隆性Ｂ细胞增生，提示ＰＣＲ法敏感性高，可能具有早期诊断价值。
Objectives To determine the relationship between MALT lymphoma and H. pylori infection and the application value of different methods in detecting their monoclonality.Methods Basing on the reinvestigation of histochemistry, Geimsa staining was used for detecting H.pylori, and immunohisto chemistry (IHC), seminested PCR amplification were used to detcte monoclonality in 31 cases of gastric MALT lymphoma. Lymphocytic gastritis diagnosed at the same period and normal gastric mucosae were selected as control. For immunohistochemistry, KiB5, L26 Antiκ, Antiλ, UCHL 1 were used as the first antibodies. For PCR amplification, primer Fr3A, Fr2A and LTH, VLTH from V and J regions respectively were used for detecing IgH gene rearrangement. Results The results showed 83.9% of MALT lymphoma were associated with H. pylori infection and 87.1% were monoclonal gene rearrangement, which being much higher than those in control group and light Chain limitation measurementby IHC. Conelusion PCR IgH gene rearrangement was better than immunohistochemical staining in the determination of monoclonality either from the viewpoint of specificity or sensitivity. In lymphocytic gastritis group, there are 26.9% monoclonal proliferation by PCR amplification unexpectedly, implying higher sensitivity of the method and possible use in the early diagnosis of the disease.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1999年第1期32-34,共3页
Chinese Journal of Digestion
