摘要
以新鲜的正常男性尿液为原料，经分离、纯化和鉴定出其分子量分别为 ２８、３５和 ５０ ｋＤ的三种蛋白质混合物，命名为Ｕ－１。采用体外试验的方法，测定了Ｕ－１对三种人癌细胞的增殖和ＤＮＡ合成的抑制作用。结果表明在低血清培养体系中对人肝癌细胞（７７２１）、人白血病细胞（Ｕ_(９３７)）和人胃癌细胞(ＭＫＮ－４５)的增殖和ＤＮＡ合成均最大抑制作用，并有很好的剂－效关系．当Ｕ－１在培养液中的浓度为０．２４ｍｇ／ｍｌ时，就能产生明显的抑制作用，达到最大抑制作用一半时 Ｕ－１的浓度（ＬＤ５０）为 ０． ２４～２ ．４ ｍｇ／ｍｌ。
The mixture of three proteins, named U-1, are isolated from normal man's urine, their molecular weight are 28, 35 and 50 kD. In vitro, U-1 can inhibit the proliferation and DNA replication of three human cancer cells. U-1 can inhibit human heptomic cell (7721 ), human Leukemic cell (U937 ) and human stomachic cell (MKN-45) in cultral liquid of low serum. The inhibition will be reinforced with the dose increasing. When the concentration of U-1 is 0. 24 mg/ml, it has significant inhibition to cancer cells, the LD50 is 0. 24-2.4 mg/ml. The action of anticancer of U-1 has something to do with the nature of antitumor in human urine.
出处
《中国生化药物杂志》
CAS
CSCD
1999年第1期7-9,共3页
Chinese Journal of Biochemical Pharmaceutics
基金
江苏省自然科学基金!(BK 97036)
南京大学现代分析中心测试基金
关键词
人尿
抗癌物质
细胞计数
蛋白质
抑制作用
Human urine, Anticancer substance, Cell counting, Incorporation of ~3H-TdR
