摘要
从自然标本中分离获得一高产甘油的菌株ＷＬ２００２５，仅发酵葡萄糖及微发酵蔗糖，能利用葡萄糖、蔗糖、乙醇生长，微利用甘油和柠檬酸，不利用肌醇、硝酸盐、赤藓醇、阿拉伯醇、甘露醇，与ＤＢＢ显色反应为阴性，可在含５００ｇ／Ｌ葡萄糖或１００ｍＬ／Ｌ醋酸的培养基中及４０℃下生长，可在水活度为０８９０～０９００的培养基中生长，出芽生殖，易形成“假丝酵母菌型”假菌丝，不进行有性生殖，线粒体ＤＮＡ的分子量为２０ｋｂ，是假丝酵母属的一个新种，定名为产甘油假丝酵母（ＣａｎｄｉｄａｇｌｙｃｅｒｏｌｇｅｎｅｓｉｓＺｈｕｇｅｓｐ．ｎｏｖ．）。
The strain WL2002 5 isolated from natural samples and overproduced glycerol from glucose was identified systematically. The WL2002 5 fermented glucose and weakly fermented sucrose; assimilated glucose, sucrose, ethanol, weakly assimilated glycerol and citrate, did not assimilated nitrate, erythritol, arabinitol, mannitol. It grew well on the slopes containing 500 g/L of glucose or 10 mL/L acetate or at the temperature up to 40℃. The minimum water activity for its growth was 0 890. It had a negative reaction with DBB and did vegetative reproduction by budding and easily formed Candida type pseudohyphae but had no sexual reproduction. The molecular weight of its mitochondrial DNA was 20 kb. We conclude that WL2002 5 is a new species of Candida and nominated it Candida glycerolgenesis Zhuge sp.nov..
出处
《微生物学报》
CAS
CSCD
北大核心
1999年第1期68-74,共7页
Acta Microbiologica Sinica
基金
国家"九五"攻关项目
关键词
产甘油假丝酵母
鉴定
发酵
甘油
Candida glycerolgenesis , Identification, Fermentation
