摘要
目的：研究ＩＬ－２基因转移对卵巢癌ＳＫＯＶ３细胞系粘附性的影响。方法：细胞对塑料基质、细胞基底膜成分的粘附实验，流式细胞术测定ＣＤ４４Ｈ的表达。结果：ＥＤＴＡ介导的细胞分离实验表明，ＳＫＯＶ３／ＩＬ－２细胞对塑料基质的粘附能力明显下降（Ｐ＜０．０１）；对基质及基底膜成分的粘附实验表明，ＳＫＯＶ３／ＩＬ－２细胞对Ⅳ型胶原和ｍａｔｒｉｇｅｌ的粘附能力下降，但对ＬＮ、ＦＮ的粘附能力无明显改变；粘附分子ＣＤ４４Ｈ的表达：ＳＫＯＶ３、ＳＫＯＶ３／Ｎｅｏ、ＳＫＯＶ３／ＩＬ－２组细胞粘附分子ＣＤ４４Ｈ阳性细胞百分率分别为７６．２％、６６．７％和３７．８％，ＳＫＯＶ３／ＩＬ－２组明显降低（Ｐ＜０．００１）。结论：ＩＬ－２基因修饰可使卵巢癌细胞系ＳＫＯＶ３粘附能力下降。
Objective:To evaluate the adhesive ability of ovarian cancer cell line SKOV3 and the IL 2 gene modified cells.Methods:EDTA induced cell detachment and cell adhesive ability to LN,FN,collagen Ⅳ and matrgel were performed.The percentage of CD44H positive cells was detected by FCM. Results:Cell adhesion tests showed that the hIL 2 modified SKOV3 cells adhered less effectively to plastic substance,collagen Ⅳ and matrigel,but there were no significant difference to LN and FN.The percentage of CD44H positive cells decreased from 76.2% of parental SKOV3 cells to 37.8% of hIL 2 gene modified cells.Conclusion:IL 2 gene modification can significantly decrease the adhesive ability of SKOV3 cells.
出处
《现代妇产科进展》
CSCD
1999年第1期22-24,共3页
Progress in Obstetrics and Gynecology
