摘要
目的探讨幽门螺杆菌感染与表皮生长因子的表达与胃癌的关系。方法应用ＰＣＲ法及快速尿素酶法检测幽门螺杆菌（ＨＰ），免疫组化法（ＡＢＣ法）检测表皮生长因子（ＥＧＦ）和表皮生长因子受体（ＥＧＦＲ），对３０例胃癌，３０例癌前病变，３０例慢性胃炎的病理组织进行了检测。结果胃癌组ＨＰ的阳性率为４６７％，癌前病变组为７６７％，胃炎组为７０％，胃癌组的ＨＰ阳性率低于癌前病变组和慢性胃炎组，胃癌组的ＥＧＦ和ＥＧＦＲ表达明显强于其它两组，癌前病变组的ＥＧＦＲ表达强于胃炎组（Ｐ＜００１）。另外，ＨＰ阳性组的ＥＧＦＲ阳性表达强于ＨＰ阴性组（Ｐ＜０００５）。结论ＨＰ感染与胃癌及癌前病变有关，ＨＰ感染可能主要作用于癌变的起始阶段；ＥＧＦ和ＥＧＦＲ在胃癌有很强的表达。
Objective To study the relationship between HP infection and EGFR expression and gastric carcinoma.Methods We measured HP by PCR,EGF EGFR by ABC immunohistochemistry of gastric carcinoma precancerous lesions and chronic gastritis,each group consists of 30 patients 30 patients.Results The HP positive rate in gastvic corcinoma,preranceous lesion and chronic gastritis is 46.7%,76.7% and 70%,respecively.The HP positive rate in gastric carcinoma group is lower than in precancerous group and chronic gastritis group (P<0 05). Expression of EGF and EGFR in gastric carcinoma group is remarkably more than in precancerous lesion group and chronic gastritis group. Expression of EGF in precancerous lesion group is obviously more than in chronic gastritis group(P<0 01) In addition, expression of EGFR in HP positive group is more stronger than in HP negative (P<0 05).Conclusion HP infection is related to gastric carcinoma and precancerous lesions and chronic gastritis;HP maybe play a role in the beginning phase of carcinoma, EGF and EGFR express obviously in gastric carcinoma.
