摘要
以高效氯氟氰菊酯为供试药剂,分别建立了烟叶基质和PBS缓冲液中高效氯氟氰菊酯的ELISA检测标准曲线。并在线性检测范围内,连续3 d添加3个浓度的高效氯氟氰菊酯,研究烟叶基质对ELISA检测的影响。结果表明,烟叶基质和缓冲液中高效氯氟氰菊酯的回收率分别在94%～157%和87%～118%,变异系数分别是6.8%～22.1%和3.5%～6.6%。这表明烟叶基质对ELISA检测干扰较大,在净化的条件下,定位为高效氯氟氰菊酯的半定量筛选检测较为合理。以空白烟叶添加适当浓度的高效氯氟氰菊酯(如设定的超标限量)为对照,间接竞争ELISA法检测待测样品,建立了烟叶中高效氯氟氰菊酯的半定量ELISA筛选方法,制定了结果判断标准。对50个高效氯氟氰菊酯施药后的烤烟烟叶同时采用ELISA法和气相色谱法(GC)分析,结果表明,ELISA法检测样本超标检出率为34%,GC法检出率为22%。以GC法为参照,ELISA法假阳性率为12%,假阴性率为0,表明该方法可以用于大量烟叶样本中的高效氯氟氰菊酯快速筛查。
The ELISA method were established for lambda-cyhalothrin in tobacco matrix and PBS respectively.To determine the matrix effect on the detection,lambda-cyhalothrin of three different concentrations were added into the samples.The results showed that overall recoveries for tobacco and PBS were 94%-157% and 78%-118%,respectively,and the coefficients of variation were 6.8%-22.1% and 3.5%-6.6%,respectively.Tobacco matrix without pretreatment had a large influence on the ELISA method.It appeared to be more suitable that ELISA method was performed as semi-quantitative method.The drug-free tobacco spiked with certain concentration of lambda-cyhalothrin were used as the control of over standard and formulated the standard of ELISA result determination.Fifty tobacco samples were detected by the ELISA and gas chromatography（GC） method.The results revealed that detection rates of over standard by ELISA and GC were 34% and 22%,respectively.Compared with GC method,the rate of false positive for ELISA method was 12% and fasle negative was 0.It was demonstrated that this semi-quantitative ELISA method could be used for the fast screening of lambda-cyhalothrin in tobacco.
出处
《江苏农业学报》
CSCD
北大核心
2010年第3期623-626,共4页
Jiangsu Journal of Agricultural Sciences
基金
国家支撑计划(2006BAK02A09-01-01)
"863"计划(2006AA068411)
云南省烟草公司(05-16)
关键词
烟叶
氯氟氰菊酯
半定量
ELISA
tobacco
lambda-cyhalothrin
semi-quantification
enzyme linked immunosorbent assay（ELISA）
