摘要
采用顶空固相微萃取(HS-SPME)和气相色谱-质谱联用(GC-MS)对不同储藏时间弱(强)筋小麦中的挥发性物质进行提取、鉴定与分析。选用复合萃取纤维二乙烯基苯-炭烯-聚二甲硅氧烷共聚物(DVB/CAR/PDMS)50μm涂层,对萃取温度、时间、样品用量和解析时间进行优化。结果表明:HS-SPME测定挥发性物质的最佳前处理条件样品量20g,萃取温度75℃,萃取时间60min,260℃条件下解析5min;经鉴定分析小麦挥发性成分主要有烃类、醛类,其次为醇类、酮类;挥发性成分总含量在储藏6个月内均呈现先降后增的趋势。弱筋小麦的烃类挥发物相对量随储藏时间延长而快速增加,醛类相对含量先降后升,而酮类和醇类相对含量则逐渐下降;强筋小麦中除烃类相对含量呈先下降而后快速增加外,其余各类挥发物含量均与弱筋小麦呈现相同的规律。储藏6个月后,变化较明显的挥发性物质有己醇、己醛、2,6,10-三甲基-十二烷、十五烷和二十烷。
By using headspace solid-phase micro-extraction （HS-SPME）,together with gas chromatography and mass spectrometry,the volatile compounds of weak-gluten （strong gluten） wheat sample were extracted,identified and analyzed.Using 50 μm divinylbenzene/carboxen/polydimethylsiloxane （DVB/CAR/PDMS） coating assembly fiber,experimental conditions including extraction time,temperature,sample amount and resolution time,have been optimized.The results showed that the optimized pretreatment conditions were sample quantity,20 g,extracting for 60 min at 75 ℃ and desorpting for 5 min at 260 ℃.The main volatile compounds in wheat by this identification method were hydrocarbons,aldehydes,and then alcohols,ketones.The total volatile matter content of wheat decreased firstly and then increased during the 6 months storage.The relative content of hydrocarbons in weak gluten wheat increased rapidly,that of aldehydes decreased firstly and then increased,and that of ketones and alcohols decreased gradually as storage time prolonging.To the strong gluten wheat,the changing law of the other volatile matter content was similar as the weak gluten wheat except that the relative content of hydrocarbons decreased firstly and then increased rapidly.The wheat volatile matters with more significant changing after 6 months normal temperature storage were 1-hexanol,hexanal 2,6,10-trimethyl-dodecane,pentadecane and eicosane.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第7期953-957,共5页
Chinese Journal of Analytical Chemistry
基金
国家"十一五"支撑项目(No.2009BADA0B05-3)资助
关键词
小麦
储藏
挥发性成分
顶空固相微萃取
气相色谱-质谱联用
Wheat
Storage
Volatile compounds
Headspace solid phase microextraction
Gas chromatography-mass spectrometry
