摘要
研究了二溴-苯基荧光酮-Mo(Ⅵ)配合物与人血清(HSA)和牛血清(BSA)蛋白作用的光谱性质、反应条件和影响因素,建立了利用金属配合物作为探针测定微量蛋白质的方法.在pH=3.8的条件下,二溴-苯基荧光酮-Mo(Ⅵ)配合物与BSA体系在530nm处有一个增强的吸收峰,且强度与BSA的质量浓度呈线性关系.在实验优化的条件下,BSA,HSA质量浓度的线性范围为2～30.0,2～25.0μg/mL;线性方程△A=0.0202ρ+0.3987,△A=0.0249ρ+0.1962,相关系数r=0.994,0.986;摩尔吸光系数ε=4.08×106,3.07×106L(/mol·cm).该方法用于HSA样品中蛋白质的测定,操作简单、灵敏度好、线性范围宽、稳定性好。
Spectral behavior,reaction conditions and effecting factors of the complex dibromophenyl-fluorone-Mo（Ⅵ） with HSA,BSA were studied.A new method for the determination of protein using the complex as a spectroscopic probe was developed.The experiment shows that the maximum absorption wavelength of the complex is at 530 nm when pH=3.8,intensity and the concentration of BSA is linear.Under the optimum condition,concentration linear range is 2～30,2～25 μg/mL,linear equation is △A=0.020 2c＋0.398 7,△A=0.024 9c＋0.196 2,correlation indexes is 0.994,0.986,molar absorptivities is 4.08× 106,3.07×106 L（/mol·cm） for BSA and HSA.It has been applied to the determination of protein in HSA samples with simple,high sensitivity,widely range of curve and well stability.
出处
《宁夏工程技术》
CAS
2010年第2期138-141,共4页
Ningxia Engineering Technology
