神经生长相关蛋白GAP-43在脊髓损伤后不同时段的表达被引量：10

Study on the Time Phases of GAP-43 Expression Following Spinal Cord Injury

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摘要目的探讨成年大鼠脊髓损伤后不同时段神经生长相关蛋白GAP-43表达的改变及其在脊髓损伤修复中的意义。方法应用改良的Allen’s法和数字化脊髓损伤模型制备仪建立大鼠脊髓损伤模型,用免疫组织化学方法检测脊髓在损伤后不同时段GAP-43的表达,分析免疫阳性细胞数和细胞的积分光密度值,资料用q检验进行统计分析。结果 GAP-43表达于脊髓神经元胞浆及突起中,在前角运动神经元中更为明显,损伤后一周内免疫反应逐渐增强,损伤后第5天积分光密度值(IOD)达到高峰(P<0.01),2周后明显下调(P<0.05)。结论脊髓损伤可能诱导损伤区GAP-43表达,在损伤后7d左右表达高峰期出现,提示其可能参与了脊髓损伤神经的生长修复过程。 Objective To investigate changes of the expression of growth associated protein （GAP-43） in adult rats with spinal cord injury in different period, and to explore its significance in spinal cord recovery. Methods Each rat were received a contusion on its spinal cord at T10 with a modified Allen＇s impaetor. Following 1, 3, 5, 7 and 14 days, the spinal cord of the rats were taken to conduct immunohistoehemical staining for GAP-43. GAP-43 positive cells were counted and the integrated optical density （IOD） was also monitored. Results The experssion of GAP-43 was shown to be in cytoplasm and processes of neurons, which being more significant in the former horn motor neu- rons. The immunohistochemical response gradully increased within the first 1 week after injury, with the IOD value reaches peak in the 5th day （P^0.05）, and decreased obviously in the next week （P^0.05）. Conclusion Spinal cord injury may induce transient up-regulation of the expression of GAP-43 in the area adjacent to injured site, and the experssion of GAP-43 being the peak in about one week after injury, indicating that GAP-43 may participate in the recovery of spinal cord after injury.

作者张奇兰李俊岑丁培培张晓

机构地区成都医学院实验技术教研室成都医学院

出处《四川解剖学杂志》 2010年第2期1-3,共3页 Sichuan Journal of Anatomy

基金四川省科技厅课题(06J13-023) 成都医学院创新实验项目(2009154)

关键词脊髓损伤神经生长相关蛋白 GAP-43 Spinal cord injury~ Growth associated protein Growth associated protein 43GAP-43

分类号 R651.2 [医药卫生—外科学]

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1Blesch A,Lu P,Tuszynski MH.Neurotrophic factors,gene therapy,and neural stem cells for spinal cord repair[J].Brain Res Bull,2002,57 (6),833-838.
2Aigner L,Arber S,Kapfhammer JP,et al.Over-expression of the neural growth-associated protein GAP-43 induces nerve sprouting in the adult nervous system of transgenic mice[J].Cell,1995,83:269-278.
3Ishii K,Nakamura M,Dai H,et al.Neutralization of ciliary neurotrophic factor reduces astrocyte production from transplanted neural stem cells and promotes regeneration of corticospinal tract fibers in spinal cord injury.J Neurosci Res,2006,84(8),1669-1681.
4Kim SU.Genetically engineered human neural stem cells for brain repair in neurological diseases.Brain Dev,2007,29 (4),193-201.
5Ludwig A,Picoc Dephio.Of 43-KD growth associated protein in primary sencory neurons leads to diminished formation spreading of growth.J Cell Biol,1993,123:417.
6Paul DS,John DH.li tubulin and GAP-43 mRNA expression in chronically injured neurons of the red nucleus after a second spinal cord injury[J].Experimental Neurology,2003,183 (2):537-547.