摘要
目的:通过研究小儿腹部恶性实体瘤(abdominal malignant tumors,AMT)术前介入治疗的临床及病理改变,探讨术前介入治疗在本病的应用价值。方法:对20例AMT患儿行介入治疗(介入组),观察分析其介入前后临床症状的变化及介入反应,同时与20例单纯手术(对照组)的AMT患儿进行手术前后临床症状的变化、疗效对照研究。对两组40例手术肿瘤标本进行多种方法的病理学对照研究,采用末端DNA转移酶dUTP缺口末端标记法(TUNEL)对两组手术标本组织切片进行细胞凋亡的检测以及用链球菌抗生物素蛋白-过氧化物酶法(S-P)测定Ki-67蛋白的表达。结果:介入组较对照组手术出血量明显减少;介入组与对照组肿瘤细胞平均凋亡指数分别为50.55±48.06和19.73±2.84,介入组显著高于对照组(P<0.001);介入组与对照组肿瘤组织Ki-67蛋白的平均表达指数分别为20.56±7.58和38.78±9.35,介入组显著低于对照组(P<0.001)。结论:术前介入治疗,能有效地提高肿瘤完整切除率,促进肿瘤细胞凋亡,降低Ki-67表达,抑制肿瘤细胞增殖活性,对防止肿瘤复发、转移及改善预后有积极意义。
Objective：To investigate the therapeutic effects of preoperative interventional therapy on abdominal malignant tumors in children by analyzing the clinical and pathological changes caused by the treatment. Methods：Twenty children patients （interventional group） with abdominal malignant tumors were received intervention therapy. Changes of clinical symptoms and reaction to the treatment of interventional group were analyzed and compared with 20 patients received surgical treatment only （surgery-only group）. Pathological findings of reseeted tumor specimens were compared between the two groups （ n = 40） by muti- measurement. Apoptosis of pathological slices in both groups was detected by terminal-deoxynucleotigyltransferase medical dUTP-X nick end labeling （TUNEL）, and the expression of Ki-67 was evaluated by streptavidin-perosidase（S-P） method. Results： Blood loss in the interventional group was significantly lower than that in the control group. The average apoptosis index （AI） of the tumor cells in the interventional group was 50. 55±48.06 , which was significantly higher than that in the control group （19. 73 ±2. 84）. The expression of Ki-67 in the interventional group and control group was 20.56±7.58 and 38.78±9.35; respectively. Interventional group was remarkably lower than control group. Conclusion： Preoperative interventional treatment can increase the chance to have the lesions completely removed,promote apoptosis of tumor cells,decrease the expression of Ki-67 and inhibit tumor cell growth. These have a positive effect on preventing relapse and metastasis of tumors and improving the prognosis.
出处
《广西医科大学学报》
CAS
2010年第3期383-385,共3页
Journal of Guangxi Medical University
基金
广西科技厅课题(No.桂科青0447040)
关键词
小儿腹部恶性实体瘤
介入治疗
临床与病理研究
abdominal malignant tumors in children
interventional treatment
clinical and pathological study
