摘要
目的:建立定性定量检测动物组织和血液中氰化钾的方法。方法:用亚硝酸钠处理待测样品后,分别以异烟酸—巴比妥酸比色法和异烟酸—吡唑啉酮比色法,对动物组织和血液中氰化钾进行定量检测;用苦味酸试纸法对氰化钾进行快速定性检验。结果:两种定量检测方法在检测氰化钾最佳反应条件下,所得结果线性良好,相关系数(r)分别为0.9999和0.9994,检出限分别为0.91μg/L和1.4μg/L;动物组织样品加标回收率的范围分别在92.32%～98.69%和93.75%～97.55%;同时验证了苦味酸试纸法对上述样品中的氰化钾也可以进行快速定性检验。结论:对检测样品采用亚硝酸钠处理后,利于氰化钾的定性定量检测;苦味酸试纸法适于基层和临床快速定性检测。
Objective：To establish qualitative and quantitative methods for detecting the potassium cyanide in animal tissues and blood samples. Methods：After treating the animal tissues and blood with sodium nitrite, the concentrations of the potassium cyanide in these samples were quantitatively determined by using isonic- otinic acid-barbituric acid colorimetry and isonicotinic acid-pyrazolone colorimetry;respectively. A qualitative detection of the potassium cyanide was done by using picric acid testing paper method. Results： The standard curves for the potassium cyanide detection were linear under the best testing conditions by using these two methods. Their linear correlation coefficients were 0. 999 9 and 0. 999 4, and their detection limits were 0.91 μg/L and 1.4 μg/L; respectively. The recovery ratios of added specimen in animal organswere 92. 32%~98. 69% and 93. 75%~97.55% respectively. The result also suggested that the acid testing paper method was effective for a qualitative detection of the potassium cyanide in 55%; plcrlc rapid the a-bove samples. qualitative and can be used for Conclusion.. Treating the tested samples with sodium nitrite beforehand is helpful for the quantitative determination of the potassium cyanide. The picric acid testing paper method rapid detection in grass roots and clinic.
出处
《广西医科大学学报》
CAS
2010年第3期354-356,共3页
Journal of Guangxi Medical University
基金
广西科技攻关项目(No.桂科攻0642001-2)
关键词
氰化钾
检测方法
动物组织
potassium cyanides detecting methods animal tissue
