摘要
纳米粒子以其较大的比表面积、良好的生物相容性而被广泛应用于分离科学领域。纳米粒子毛细管电色谱(NPCEC)是纳米材料技术与毛细管电泳/电色谱(CE/CEC)技术相结合的产物。NPCEC技术是将不同基质的功能化纳米粒子加入电泳的运行缓冲液中,纳米粒子不仅可以通过动态吸附于毛细管壁而改变(或逆转)电渗流,还可作为准固定相(PSPs)参与样品在柱内的分配和保留,从而提高柱效和改善分离的选择性。该技术因无需填充和柱塞制备等步骤而日益受到关注。此外,NPCEC技术还可实现良好的柱更换,避免了进行复杂样品分析时所造成的柱污染。该文重点讨论在NPCEC实验中常用的几种纳米粒子的合成及应用,包括聚合物纳米粒子、二氧化硅纳米粒子、金纳米粒子、碳纳米管和树状聚合物,并对其发展前景进行了展望。
Nanoparticles are widely used in separation science,due to their favorable surface-to-volume ratios as well as good biocompatibility.Nanoparticle capillary electrochromatography（NPCEC） is a hybrid of the nanomaterials technique and capillary electrophoresis/electrochromatography（CE/CEC）.In NPCEC technique,different types of nanoparticles were added into the running buffer,which can be kinetically Absorbed onto the inner surface of capillary to change（or reverse） EOF.As separation media,nanoparticles can also participate in the separation process acting as pseudostationary phase（PSP）to improve the separation efficiency and selectivity.NPCEC benefits from neither retaining frits nor complicated packing procedure,which attracted a considerable attention in separation science.Furthermore,the pollution by complex matrix can be efficiently avoided for one-time use of the stationary phase allowing fast regeneration of column,Absence of stationary phase carry-over effects,and an easily exchanged column.In this review,the synthesis and applications of polymer nanoparticles,silica nanoparticles,gold nanoparticles,carbon nanotubes and dendrimers as PSPs will be disscused in detail.
出处
《分析测试学报》
CAS
CSCD
北大核心
2010年第6期638-644,共7页
Journal of Instrumental Analysis
基金
国家自然科学基金青年科学基金资助项目(20505011)
关键词
纳米粒子
毛细管电色谱
准固定相
综述
nanoparticle
capillary electrochromatography
pseudostationary phase
review
