摘要
目的：研究我国肿瘤高发区饮食污染霉菌毒素－杂色曲霉素（ＳＴ）对人免疫机能的可能影响。方法：流式细胞术（ｆｌｏｗｃｙｔｏｍｅｔｒｙ，ＦＣＭ）、形态学观察及ＤＮＡ电泳。结果：ＳＴ可诱导体外培养的人外周血淋巴细胞发生凋亡，并且在一定时间（２～４８ｈ）及剂量（０１２５～２．０ｍｇ／Ｌ）范围内呈正相关关系。光镜及透射电镜观察均发现了凋亡细胞典型的形态学变化，如核染色质凝集边集，凋亡小体形成等；ＤＮＡ琼脂糖凝胶电泳也出现特征性的ＤＮＡＬａｄｄｅｒ条带。结论：ＳＴ可诱导人外周血淋巴细胞发生凋亡。
AIM:To explore the possible impacts of sterigmatocystin (ST) on human immunological system. METHODS:Flow cytometric (FCM) analysis, morphological observation and DNA electrophoresis. RESULTS:FCM analysis revealed that the apoptotic rates of all the lymphocytes in ST treated groups were significantly higher than those in their corresponding control groups. Significant dose-effect and time-effect correations could be found between apoptotic rates and ST concentrations in the range from 0.125 mg/L to 2 mg/L as well as ST treating time from 2 h to 48 h in the same concentrations (2 mg/L). Further works showed that under light and electron microscope, chromatin condensation and margination alongside nuclear membrane, nuclear membrane disintegration, nuclear fragmentation as well as apoptotic bodies were found in ST treated cells in semi-ultrathin and ultrathin sections. While in agarosegel electrophoresis, a characteristic “ladder” pattern caused by products of DNA degradation was also found in ST treated cells. CONCLUSION:ST could induce apoptosis in human peripheral blood lymphocytes in vitro . Thus it might have some negetive impacts on human immunity.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第1期33-35,共3页
Chinese Journal of Pathophysiology
基金
河北省自然科学基金
