摘要
目的观察杂色曲霉素(ST)对外侧隐窝处室管膜细胞、神经元、神经纤维和血管内皮细胞的影响。方法BALB/c小鼠,ST单次灌胃,分别于灌胃后1、2、4、81、6 h处死动物,用透射电镜观察外侧隐窝室管膜细胞、神经元、神经纤维和血管内皮细胞的结构变化。结果ST灌胃后1 h血管内皮细胞吞饮囊泡开始增多,2 h组室管膜细胞的顶浆分泌增多,部分神经髓鞘变性;4 h和8 h组神经纤维广泛变性,神经元线粒体变性、核切迹和空泡出现;16 h组神经元胞浆内线粒体变性更加明显,并出现脂褐素。结论单次灌胃后,ST即可引起外侧隐窝处室管膜细胞、神经纤维、神经元和内皮细胞的改变,但ST主要是对神经纤维和神经元的损伤。
Objective To investigate the effects of sterigmatocystin（ST） on the ependymal cells, neurons and endothelial cells of blood vessel of the lateral recess of the fourth ventricle in BALB/c mice in vivo after single oral administration. Methods BALB/c mice were treated intragastrically by gavage with ST 3000 μm/kg. The mice were killed 1 hour, 2 hours, 4 hours, 8 hours, 16 hours after oral administration respectively for observing ependymal cells, neurons and endothelial cells of blood vessel of the lateral recess with transmission electron microscopy （TEM）. Results After oral administration of ST pinocytosis vesicles began to increase in endothelial cells of blood vessel in 1 hour, apocrine of ependymal cells began to increase and myelin sheath degeneration appeared in 2 hours, extensive myelin sheath degeneration and mitochondrion degeneration and incisure and vacuoles in nucleus of neuron were found in 4 and 8 hours. Lipofuscin was found in cytoplasm of neuron 16 hour. Conclusion Oral ST exposure in mice could cause myelin sheath degeneration and damaged changes of neurons.
出处
《解剖学报》
CAS
CSCD
北大核心
2006年第2期149-152,共4页
Acta Anatomica Sinica
基金
教育部科学技术研究重点项目(205021)
河北省自然科学基金项目(301350)资助
