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神经干细胞移植抑制缺血再灌注大鼠脑内星形胶质细胞的激活和炎症反应 被引量：2

Neural stem cell transplantation restrains astrocyte activation and inflammation in brain of rats with cerebral ischemia-reperfusion
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摘要 目的观察神经干细胞(NSCs)移植对脑缺血再灌注损伤大鼠脑组织胶质纤维酸性蛋白(GFAP)和炎症因子(IL-1β、TNF-α)表达的影响,探讨其可能的作用机制。方法大鼠随机分为脑组织缺血再灌注损伤模型组(MCAO组)和神经干细胞移植组(MCAO+NSCs组),每组16只,干细胞移植后进行神经功能损害评分(NSS),采用蛋白质免疫印迹法和免疫组织化学法检测脑组织GFAP表达,蛋白质免疫印迹法检测脑组织IL-1β、TNF-α的表达。结果移植后2周、4周神经功能评分显示MCAO+NSCs组显著优于MCAO组(P<0.05);MCAO+NSCs组Nestin、Brdu阳性细胞数多于MCAO组(P<0.05),GFAP阳性细胞数显著少于MCAO组(P<0.05);MCAO+NSCs组IL-1β、TNF-α表达水平显著低于MCAO组(P<0.05)。结论 NSCs移植可能通过抑制脑组织星形胶质细胞的激活、降低脑内炎症反应对大鼠缺血再灌注损伤脑组织发挥保护作用。
作者 赵宇 谢鹏 朱晓峰 蔡志友
机构地区 重庆医科大学附属第一医院神经内科 佳木斯大学神经科学研究所
出处 《第二军医大学学报》 CAS CSCD 北大核心 2010年第6期678-681,共4页 Academic Journal of Second Military Medical University
关键词 神经干细胞移植 大脑中动脉阻塞 胶质纤维酸性蛋白 炎症
分类号 R743 [医药卫生—神经病学与精神病学]
  • 相关文献

参考文献13

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二级参考文献11

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共引文献17

同被引文献31

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二级引证文献7

第二军医大学学报
2010年 第6期

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