摘要
目的通过检测大鼠全脑缺血后再灌流血浆及脑组织中内皮素的含量变化，初步探讨了内皮素与脑秧血再灌流后海马组织损伤的关系。方法利用四血管阻断法形成大鼠全脑缺血模型，在全脑缺血10min后再灌流6h、24h、72h以及120h后分别检测血浆及脑组织中内皮素的含量。结果大鼠全脑缺血再灌流后血浆及脑组织中内皮素含量明显升高，特别是海马组织中内皮素的增高最为明显。内皮素的增高在脑缺血后24－72h达到最高峰，120h有下降趋势。结论内皮素的改变与脑缺血再灌流引起的海马神经元迟发性坏死有密切的关系。
Objective The chang of EF -1 concentrations was detected in the Plasma and brain tissuc after theglobal ischemia and reperfusion of rat and the relationship of ET - 1 and the hippocampal injury after reperfusion was invesugated. Methods Tfe four- vessel occlusion (4- VO) model was made. as described by Pulsinelli-Brierley in SpragueDaewley rats. After 10 minutes of ischemia the plasma and brain tissues' concentrations of ET- 1 was detected the repenusion of 6 hr, 12 hr, 24hr, 72 hr and 120 hr respectively. Results The concentrations of ET-1 in the global ischemic braintissues and plasma significantly increased after transient cerebral ischemia. And the concentrations became highe with theprolongation of ischemia. It was for tha the concertrations of ET -1 in the hippocampus were much higher than tha inthe corte after the global ischemia Conclusion It might confirm that the marked increase of ET-1 of in the brain tissues andplasma was associated with global ischemic events. And ET- 1 might play some the in the delayed neuron death of hippocampal neurons after the global ischemia and reperfusion.
出处
《广东医学》
CAS
CSCD
1999年第1期4-5,共2页
Guangdong Medical Journal
关键词
内皮素
大鼠
神经元坏死
脑缺血
再灌注损伤
Endothelin Rats Global ischemia and reperfusion Delayed neuron death
