摘要
目的：将抗人原发性肝癌（ＰＬＣ）单抗ＴＩＧＴＣⅢ，用胃蛋白酶法消化成Ｆ（ａｂ′）２片段，标记１３１Ｉ后进行裸鼠人肝癌移植瘤的体内定位研究。方法：采用胃蛋白酶消化法，进行裸鼠体内核素显像。结果：消化产率为３１７％±２４％，经细胞结合实验证明Ｆ（ａｂ′）２具有良好的免疫活性，抗体片段进入裸鼠体内后，与完整抗体比，进入肿瘤的速度快，生物学分布特异性高，定位指数上升，血清本底下降迅速，生物半衰期明显缩短。结论：Ｆ（ａｂ′）２与全抗体比较可使成像时间提前。
Objective:In order to realize the immunoimmaging with F(ab′) 2 fragment of monoclonal antibody (TIGIC Ⅲ) for PLC.Methods:Monoclonal antibody(TIGTC Ⅲ) against human primary liver cancer(PLC) was digested into F(ab′) 2 fragment by pepsin digestion.After labelled with 131 I and 125 I radioiodinated F(ab′) 2 was injected into nude mice bearing tumor xenografts of PLC via tail vein and radioimmunoimagine was performed.Results:The experiment results showed that the yield of pure F(ab′) 2 was 31.7%±2.4%,the immunoreactivity of F(ab′) 2 based on the cell binding studies was retained well.After the injection of 131 I F(ab′) 2 into PLC tumor bearing nude mice,photoscintigraphy was performed at intervals of 12 h.The xenografts were visualized distinctly during 24～96 h.Conclusion:The ratio of tumor to non tumor(T/NT) was higher than those of the intact IgG in all tissues.Xenografts of PLC in nude mice acumulated TIGTC Ⅲ.The excretion of radioactivity was decreased repidly.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第1期35-36,39,共3页
Chinese Journal of Immunology
