摘要
目的 了解CD3AK细胞在双功能单抗介导下对人原发性肝癌 (PLC)的杀伤作用。方法 用二次杂交瘤方法制备得双功能单抗 (GP95×CD3) ,将该单抗激活人淋巴细胞 ,分别测定后者对PLC细胞的体内外杀伤效应。结果 GP95×CD3能显著提高T细胞与癌细胞的结合率 ,对靶细胞的杀伤具有较强的特异性。体外试验明显增加细胞毒效应 ,裸鼠体内试验具有显著抑制肝癌生长的作用。结论 GP95×CD3在体内外均可介导CD3AK细胞对PLC的杀伤作用。
Objective In the present study we tested whether human CD3AK cells from normal donors can be targeted against human primary liver cancer (plc) cells and block growth of an established tumor in nude mice. For targeting we used bispecific monoclonal antibodies reacting with CD3 on the T cells and with GP95 selectively expressed by plc cells. Methods Bispecific antibodies (GP95×CD3) was produced by fusion of the TIGTC Ⅲ and 12F6 hybridomas. CD3AK cells were obtained from normal dondrs by culturing PBLS with GP95×CD3 and IL 2 anti tumor effects were performed by cytotoxicity assays in vitro and anti tumor assay in vivo . Results CD3AK cells proliferation was enhanced significantly by bispecific monoclonal antiboby. GP95×CD3 efficiently promoted targeted cell lysis in vitro and tumor growth inhibition in vivo .Conclusion It had been shown that bispecific monoclonal antibody (GP95×CD3) by hybrid hybridoma technology can target CD3AK cell to lyse PLC cells in vitro and to inhibit the growth of PLC in vivo .
出处
《免疫学杂志》
CAS
CSCD
北大核心
2002年第B06期4-6,共3页
Immunological Journal
基金
国家自然科学基金资助项目 (39370 6 2 0 )
