摘要
目的建立实验性自身免疫性脑脊髓炎（ＥＡＥ）动物模型，探讨口服自身抗原诱导免疫耐受对大鼠ＥＡＥ的防治作用。方法在普通Ｗｉｓｔａｒ大鼠经１次足跖真皮内注射完全弗氏佐剂－豚鼠全脊髓匀浆或完全弗氏佐剂－髓鞘碱性蛋白乳剂加百日咳疫苗诱发ＥＡＥ疾病；另在大鼠致炎前及发病后口服髓鞘碱性蛋白（ＭＢＰ），观察其对ＥＡＥ的防治作用。结果在Ｗｉｓｔａｒ大鼠成功地诱发了ＥＡＥ，发病率将近９０％。大鼠在致炎前口服ＭＢＰ，可明显推迟ＥＡＥ发病时间，降低ＥＡＥ发病率，使神经组织病理改变明显改善。大鼠发生ＥＡＥ后给予ＭＢＰ，可明显控制发病动物的病情，使病程缩短及减轻患鼠神经组织的炎症程度。另外，在耐受鼠由ＭＢＰ引起的迟发型超敏反应（ＤＴＨ）以及体外针对ＭＢＰ的淋巴细胞增殖反应也明显受到抑制。结论口服ＭＢＰ可引起特异性的免疫耐受。
Objective To establish on animal model of experimental autoimmune encephalomyelitis (EAE), and to investigate the therapeutic effect of orally administrated myelin basic protein (MBP) on EAE. Methods With guinea pig spinal cord homogenate in complete Freund's adjuvant (CFA GPSCH) or MBP in complete Freund's adjuvant (CFA MBP) and Bordetella pertussis vaccine (BPV) was injected intradermally to induce EAE in Wistar rats. To investigate the suppression of clinical and histological manifestations of the disease when animals were fed with MBP before or after disease induction. Results With CFA GPSCH or CFA MBP and BPV, we succeeded in the induction of EAE in Wistar rats, and the incidence of EAE was more than 90%. The clinical expression of the disease was suppressed whether animals were fed with MBP before or after sensitization, the incidence of EAE was dramatically decreased in animals which were fed with MBP before disease induction, and the duration of disease was shortened significantly in animals which were fed with MBP after onset of the disease. Histological examination revealed a marked reduction of perivascular infiltrates in rats fed with MBP either before or after sensitization to MBP. In addition, DTH responses to whole MBP were suppressed and the suppression of in vitro proliferative responses to MBP were observed to be antigen specific. Conclusions EAE can be prevented and cured by oral administration of MBP to induce specific immunologic tolerance.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1999年第2期144-147,共4页
Chinese Journal of Microbiology and Immunology
基金
国家教委博士点基金
关键词
髓鞘碱性蛋白
自身免疫性
脑脊髓炎
治疗
Myelin basic protein
Autoimmune encephalomyelitis
Autoimmune disease
Immune tolerance
