摘要
目的 探讨参附注射液（SF）预处理对犬心肌缺血-再灌注损伤（MI/RI）的保护作用.方法 20只健康成年杂种犬随机分为低剂量SF组（2 ml/kg,SF2组）、高剂量SF组（4 ml/kg,SF4组）、生理盐水模型组（N组）和假手术组（S组）,每组5只.开胸后结扎犬冠状动脉左前降支,建立在体急性心肌I-R模型.用放射免疫法检测术前（T1）、冠脉结扎前（T2）、再灌注前（T3）、再灌注1 h（T4）和再灌注2 h（T5）血清肌钙蛋白T（cTnT）含量,用免疫组化法检测T5心肌组织核因子（NF）-κB和热休克蛋白70（HSP70）的光密度值.结果 与S组比较,SF2、SF4和N组心肌HSP70和NF-κB、T2～T5时血清cTnT的含量显著升高（P〈0.05）.与N组比较,SF2、SF4组血清cTnT的含量有所降低,心肌HSP70的光密度值高,NF-κB的光密度值减低（P〈0.05）.结论 SF预处理可能通过降低血清中的cTnT的含量、增强心肌HSP70的表达和减低NF-κB的活性而对I-R损伤心肌产生保护作用.
Objective To study the protective effects of shenfu injection（SF） on myocardial ischemia-reperfusion（I-R） injury in dogs. Methods Twenty adult healthy cross bred dogs were randomized into four grouds of SF 2 ml/kg group（SFz ）, SF 4 ml/kg group（SF4 ）, sham operated group（S） and normal saline（NS） control group（N）, with 5 dogs each SF or NS were injected before operation. The myocardial I-R injury model was established by ligating the left anterior descending coronary artery. The contents of serum eadiac troponin T （cTnT） were measured before operation and ligation,at 30 rain after ligation, 1 h and 2 h after referfusion by radioimmunity. The expressions of heat shock protein 70（HSP70） and nuclear factor-KB（NF-KB） in the cardiac muscle tissues were measured by immunohistochemistry at 2 h after referfusion. Results Compared with group S, serum contents of cTnT, HSP70 and NF-KB of the other three froups were significantly higher （P〈0.05）. Compared with group N, serum levels of cTnT were lower, the expression of HSP70 was increased and the expression of NF^B was lower （P〈0.05）. Conclusion SF has a protective effect on the myocardial I-R injury, which may be related to its function of decreasing the levels of eTnT, and decreasing the expression of HSP70, and lowering the activity of NF-kB.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2010年第5期433-435,共3页
Journal of Clinical Anesthesiology
