摘要
目的探讨三七总皂甙(total saponins of panax notoginseng)在骨折愈合中的作用,为其临床应用提供实验依据。方法在36只雄性SD大鼠左桡骨形成骨折模型,后随机分成对照组和PNS组,予以PNS100mg/(kg.d)腹腔内注射,对照组予以等量盐水。在骨折后7天、14天、21天X线观察骨折愈合及骨痂形成情况;HE染色了解骨痂形成情况;评价全血黏度和血浆黏度水平。结果X线显示PNS组骨痂形成明显加快;HE染色显示PNS组骨折断端成骨细胞数目及骨痂增多;PNS组全血黏度和血浆黏度降低。结论PNS可低全血黏度及血浆黏度,改善血液流变性和骨折部位的血供,从而利于骨折愈合。
ObjectiveTo investigate the effects of total saponin of panax notoginseng on bone fracture healing. Methods36 rats were divided into PNS group and control group.The left radius fracture model was used in this experiment.PNS 100mg/（kg·d） was intraperitoneal injections in rats of PNS groups and same dose isotonic saline was treated in control group.Rats in control group and PNS group were sacrificed and necropsied at 7,14 and 21day （n=6） .The tissue or callus around fracture site were obtained for histological examnation and callus measurement.The formation of callus was monitored by X- ray.The viscosity of whole blood and plasma was also studied. ResultsThe formation and thickness of callus were enhanced by PNS.HE staining showed that the chondrocytes,osteoblasts and callus appeared earlier and more in PNS groups than control （P〈0.05）.The level of viscosity of whole blood and plasma was decreased by PNS.ConclusionPNS can increase callus content and promote the process of the bone fracture healing.Down-regulation of the viscosity of whole blood and plasma is the potential mechanism of PNS on accelerating the bone healing.
出处
《医学研究杂志》
2010年第6期69-71,共3页
Journal of Medical Research
基金
中国博士后课题基金项目(20090450913)
广东省中医药局科研基金项目(2008163)
关键词
三七总皂甙
骨折
骨折愈合
鼠
Total saponins of panax notoginseng,PNS, Bone fracture,Fracture healing Rats
