摘要
目的本文作者观察了ｒｈＴＰＯ和ｒｈＧＣＳＦ对急性放射病猴的治疗效果，旨在为造血因子的临床应用提供理论依据。方法正常雄性成年猕猴２０只，随机分为ｒｈＴＰＯ１０μｇ·ｋｇ－１·ｄ－１、ＧＣＳＦ１０μｇ·ｋｇ－１·ｄ－１、ＴＰＯ＋ＧＣＳＦ和照射对照４组。动物照射的吸收剂量为５０Ｇｙ（３００ｋＶＸ射线，吸收剂量率为０２０～０２２Ｇｙ／ｍｉｎ）；给药方法均为皮下注射，连续给药２１天。结果①外周血小板：照后第７天ＴＰＯ治疗组开始下降，第１０天达最低值（４５％，以照前值为１００％，下同），第１４天达照前值水平，第２１天达最高值（３１１％），第３０天仍为２６３％。ＧＣＳＦ治疗组第９天仅为照前值的１０％，照后第３０天接近照前水平（９２％）。对照组照后第７天开始迅速下降，第１６天达最低值（９％），仅为ＴＰＯ治疗组的５９％（Ｐ＜００１），第３０天（８０％）仍未恢复到照前值水平。②外周血网织红细胞百分比：４组动物照后均迅速下降，ＴＰＯ治疗组于照后第８天达最低值（１１％），低于照前值５０％的时间为８天，第１２天开始迅速回升，第２０天达最高值（４０２％）。ＧＣＳＦ治疗组及对照组动物下降程度更为迅速，第９天分别为照前?
Objective\ This report describes the therapeutic effects of recombinant human thrombopoietin(rhTPO) and recombinant human granulocyte colony stimulating factor(rhG CSF) on acute radiation sickness in monkeys.Methods rhTPO(10μg·kg -1 ·d -1 ,sc,on days 1 21 after TBI)or/and rhG CSF(5μg·kg -1 ·d -1 ,sc,on days 1 21 after TBI) were administered to rhesus monkeys with 5.0Gy X irradiation.Results rhTPO promoted platelet and reticulocyte recovery,resulting in less profound nadirs and a rapid recovery to normal levels.Platelet transfusions were not required in contrast to controls,while hemoglobin levels stabilized rapidly.TPO treatment did not influence neutrophil counts, rhG CSF stimulated neutrophil regeneration and had no effect on platelet levels.Simultaneous administration of rhTPO and rhG GSF were as effective as rhTPO alone in preventing thrombopenia,although the platelet level did not rise to the supranormal level seen with TPO alone. The neutropils were greatly augmented in number,compared to G CSF treatment alone,resulting in a less profound nadir and much earlier recovery,which was similarly observed for monocytes,CD 11 b +,CD + 16 and CD + 56 cell reconstitution. Conclusion These data show that rhTPO and rhG GSF are potent stimulators of hematopoiesis in monkeys with radiation sickness.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
1999年第1期34-38,共5页
Chinese Journal of Radiological Medicine and Protection
关键词
血小板生成素
RHG-CSF
急性放射病
GM-CSF
Thrombopoietin\ \ Granulocyte colony stimulating factor\ \ Acute radiation sickness\ \ Hematopoietic growth factor
