摘要
目的探讨放射损伤、烧伤与放烧复合伤后小鼠小肠粘膜免疫变化及其用细胞因子调控后与肠源性感染的关系。方法采用斑点杂交、免疫组化、免疫酶联吸附实验、细菌培养等方法。结果①三类伤组伤后均出现小肠粘液ｓＩｇＡ含量显著降低，浆细胞数量减少，肠道细菌移居增多，肠系膜淋巴结ＩＬ４ｍＲＮＡ表达降低，尤以复合伤组最重。②用ＩＬ４蛋白调控后，三类伤组肠道浆细胞的数量增多，小肠粘液中ｓＩｇＡ含量相应增加，肠道细菌移居减少。结论伤后粘液中ｓＩｇＡ的降低与肠源性感染的发生密切相关，其原因与分泌ｓＩｇＡ的淋巴细胞数量减少和功能障碍有关；而促进粘膜免疫功能恢复。
Objective\ To explore the change of intestinal mucosal immunity and its relation with enterogenous infection after irradiation or burn alone or both combined. Methods Spot hybridization, immunohistochemistry, ELISA and bacterial culture were used. Results (1) After the three modes of injury the quantity of mucosal sIgA and numbers of IgA secreting plasma cells in the intestine were all significantly decreased, enterogenous infection was increased, the IL 4mRNA expression of mesenteric lymphocytes was reduced, and the damage in the combined radiation burn group was more severe. (2) After treatment with IL 4, the number of plasma cells and the quantity of IgA secretion were obviously increased and the incidence of enterogenous infection was reduced. Conclusion Enterogenous infection is highly related with depression of sIgA mainly owing to reduction of quantity and function of IgA secreting plasma cells. It is beneficial to enchance the recovery of mucosal immunity and diminish incidences of entergenous infection.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
1999年第1期15-17,共3页
Chinese Journal of Radiological Medicine and Protection
基金
国家自然科学基金
关键词
放烧复合伤
小肠粘膜免疫
肠源性感染
放射损伤
Combined radiation burn injury\ \ Mucosal immunity \ \ Enterogenous infection
