摘要
研究假肥大型肌营养不良（ＤＭＤ）病人肌酸激酶同工酶（ＣＫ－ＭＢ）及其亚型的变化，为早期诊断和判定病情提供依据。方法采用不连续缓冲体系电泳法将肌酸激酶分离成同工酶及亚型，荧光扫描。结果ＤＭＤ病人不同年龄组间ＣＫ－ＭＢ同工酶有显著性差异（Ｐ＜０．０５），病程早期ＣＫ－ＭＢ同工酶与参考值间有显著性差异（Ｐ＜０．０５），病程晚期，ＣＫ－ＭＢ同工酶与参考值间却无显著性差异，但ＣＫ－ＭＢ亚型值不同年龄组均与参考值间有显著性差异（ＰＭ０．０５）。结论ＤＭＤ病人病情的变化与肌酸激酶及ＣＫ－ＭＢ同工酶不呈正相关，ＣＫ－ＭＢ亚型的变化与ＤＭＤ病人的病情呈正相关。ＣＫ－ＭＢ亚型可作为ＤＭＤ病人诊断和判定病情的重要指标。
To study the changes of creatine kinase isoenzyme(CK-MB)and its isoforms in patient with DMD for the purpose of early diagnosis and evaluation, a discontinuous buffer system was used. CK-MB isoforms was separated byelectrophoresis, under constant current and low voltage,fluorescence scanning. A statistically significant changes ofCK-MB in younger age group of subjects when compared with control group (P<0.05),and also the statisticallysignificant among these three age groups of the subject (P<0. 05),but there is no statistically significant in the later stage of the subject (P> 0. 05 ). However,a statistically significant changes of CK-MB isoforms in all the subjectwas found when compared with control group (P<O. 05). The creatine kinase isoenzyme (CK-MB)can not reflectthe real condition of patient with DMD, but CK-MB isoforms can. The study of CK-MB isoforms provides usefulinformation in early diagnosis and evaluation for DMD patient.
出处
《上海医学检验杂志》
1999年第1期24-26,共3页
Shanghai Journal of Medical Laboratory Sciences
关键词
假肥大型
肌营养不良
肌酸激酶同工酶
电泳
Duchenne muscular dystrophy (DAD) Creatine kinase isoenzyme (CK-MB)CK-MB isoforms Electrophoresis.
