摘要
目的:研究荷瘤小鼠脾脏中髓系抑制性细胞(Myeloid-derived suppressor cells,MDSCs)比例的变化及在组织中的分布,探讨其在肝癌免疫逃逸中的作用。方法:采用肝癌细胞原位移植法建立小鼠原位肝癌模型,流式细胞仪检测正常小鼠和荷瘤小鼠7、14、21天脾脏中MDSCs的比例,免疫组织化学染色对其进行定位分析。结果:荷瘤小鼠脾脏中MDSCs的比例比正常小鼠显著增高(P<0.05),而且随着荷瘤时间的延长,MDSCs的比例逐渐增加。正常小鼠脾脏中少量的MDSCs散在分布于红髓区,荷瘤后大量MDSCs主要聚积在边缘区及白髓区的动脉周围淋巴鞘。结论:小鼠脾脏中MDSCs的比例与肿瘤进展相关,且主要分布在脾脏胸腺依赖区。
Objective：The changes and distribution of myeloid-derived suppressor cells （MDSCs） in spleen of tumor-bearing mice was studied,and its function in tumor escape was investigated.Methods：Orthotopic transplantation tumor model of hepatocarcinoma in mice was established.At the 7th,14th,21st day after tumor implantation,the proportion of MDSCs in spleen was tested by flow cytometry.The localization of MDSCs and semi-quantitative analysis was conducted by immunohistochemistry.Results：The proportion of spleen MDSCs in tumor-bearing mice was significantly higher than in normal mice （P0.05）.Accompanying with tumor progression,the proportion of spleen MDSCs increased gradually.Under resting conditions,a few MDSCs were mainly localized in the red pulp of the spleen.A dramatic increase in spleen MDSCs was oberseved in tumor-bearing mice and tightly accumulated in the marginal zones and periarteriolar lymphatic sheaths.Conclusion：The proportion of spleen MDSCs in mice is associated with tumor progression,and mainly localize at thymus-dependent area.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2010年第6期507-509,513,共4页
Chinese Journal of Immunology
关键词
髓系抑制性细胞
免疫逃逸
荷瘤
肝癌
Myeloid-derived suppressor cells
Immune escape
Tumor-bearing
Hepatocarcinoma
