摘要
利用自制的气升式内环流生物反应器进行青蒿（ArtemisiaannuaL．）毛状根多层培养生产青蒿素。毛状根培养物在培养过程中均匀分布在生物反应器的筛网间，或以不锈钢网为附着点向四周生长，在25℃和12h／d光照周期下，经20d分批培养获得生物量干重22．57g／L，青蒿素产量374．4mg／L，并对培养过程中蔗糖、磷酸盐、硝酸盐和氨盐消耗的动力学进行了分析。
A self-made internal loop airlift bioreactor was used for production of artemisinin by Artemisia annua L. hairy root multi-plate culture. The hairy root cultures grew evenly either between the two meshes or attaching to the stainless steel meshes in the bioreactor. Under the optimal conditions of 12 h/d light cycle and25℃, the dry weight and artemisinin yield reached 22. 57 g/L and 374 .4 mg/L respectively after 20 days.Meantime, the kinetics of sugar, phosphate, nitrate and ammonium uptake during the course of culture werestudied.
基金
国家"九五"重点攻关项目
关键词
青蒿
生物反应器
毛状根
青蒿素
动力学
Artemisia annua, Internal loop airlift bioreactor, Hairy root, Artemisinin, Kinetics
