摘要
目的探讨启膈散治疗食管癌作用机制。方法 100只小鼠随机分为10组,启膈散1、2、3、4、5、6、7组各10只,刀豆素A(ConA)组10只,脂多糖(LPS)组10只,磷酸缓冲液(PBS)组10只。四甲基偶氮唑盐(MTT)法、L929细胞毒生物法检测各组小鼠单核巨噬细胞代谢、吞噬功能及分泌肿瘤坏死因子-α(TNF-α)的功能。结果启膈散各组在促进小鼠单核巨噬细胞增殖、增强单核巨噬细胞吞噬功能及分泌TNF-α的功能方面与ConA组、LPS组比较差异均有统计学意义(P<0.05),启膈散组优于ConA组和LPS组,且存在明显量效关系。结论启膈散可通过促进小鼠单核巨噬细胞增殖、增强单核巨噬细胞吞噬功能及分泌TNF-α的功能从而达到抑制肿瘤生长的作用。
Objective To investigate the anticancer effect and mechanism of Qigesan.Methods The proliferation and phagocytosis of BALB/C＇s monocyte cells was analyzed using MTT assay with and without the treatment of Qige San in concentrations of 15.6（～）500mg/ml.Tumour necrosis factor（TNF）activity was measured by an L929 cytotoxicity bioassay.Results Qige San can significantly promote the proliferation of BALB/C＇s monocyte cells,increase the phagocytosis of BALB/C＇s monocyte cells,stimulate the exudation of TNF-α by BALB/C＇s monocyte cells.Conclusion Anti-tumour effect of Qigesan is closely related to improving function of immunocyte on BALB/C＇s monocyte cells.
出处
《河北中医》
2010年第4期603-605,共3页
Hebei Journal of Traditional Chinese Medicine
基金
2005年沧州市科学技术研究与发展指导计划(编号:05ZD21)
