摘要
目的：研究藏药忍冬果水提物（FLM）对小鼠腹腔巨噬细胞吞噬功能及分泌细胞因子的影响。方法：运用吞噬中性红试验观察FLM对小鼠腹腔巨噬细胞吞噬功能的影响，用小鼠腹腔巨噬细胞分泌物对小鼠胸腺细胞增殖及对L929细胞杀伤作用的影响分别测定IL-1及TNF-α的活性，用RT-PCR技术观察药物对小鼠腹腔巨噬细胞TNF-α mRNA和IFN-γ mRNA表达的影响。结果：FLM在1～100μg·mL^-1剂量内对小鼠腹腔巨噬细胞吞噬功能有明显促进作用，同时能诱导巨噬细胞分泌IL-1及TNF-α，并促进细胞因子，INF-α mRNA和IFN-γ mRNA的表达。结论：FLM对小鼠腹腔巨噬细胞吞噬功能及分泌细胞因子都有促进作用。
Objective： To explore the effects of traditional Tibetan medicine, Fructus Lonicerae microphyllae （FLM）on phagecytosis and cytokines production of murine macrophages, Method： The phagecytosis of murine macrophages was analyzed by neutral red phagecytosis assay. The activities of IL-1 and TNF-α were measured by biological methods. The mRNA of TNF-α and INF-T expressed by macrophages was detected by RT-PCR. Result： The phagecytosis of murine macrophages was significantly enhanced by FLM at a concentration from 1μg·mL^-1 to 100 μg· mL^-1 and the secretions of IL-1 and TNF-α from macrophages were markedly induced by FLM. Meanwhile, FLM also increased the expression of TNF-α mRNA and INF-γ mRHA from macrophages in vitro, Conclusion： FLM could promote phagecytosis and cytokines production of murine macrophages.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第2期145-148,共4页
China Journal of Chinese Materia Medica
关键词
忍冬果
巨噬细胞
吞噬作用
细胞因子
Fructus Lonicerae micmphyllae
macmphage
phagecylosis
cytokine
