摘要
根据鸭疫里默氏杆菌16SrRNA基因的保守序列设计特异性引物和TaqMan探针,建立了该菌的荧光定量PCR检测方法。利用该方法分别对自然和人工感染病例不同脏器的细菌进行定量检测,并对临床疑似阳性病例进行诊断。试验证明,该方法具有敏感性高、特异性好、稳定性强的特点;在自然感染和人工感染的鸭病例中,脑组织和心脏的细菌含量最高。该检测方法能够很好地应用于鸭疫里默氏杆菌的快速检测。
A pair of primers and one TaqMan probe were designed in conservative region of the 16S rRNA gene of Riemerella anatipestifer （RA）,and a fluorescent quantitative PCR （FQ-PCR） method was successfully established for detection of RA.The different quantity of RA from either naturally or artificially infected organs was detected by the method respectively,whereby clinically suspected positive samples were diagnosed.The results showed that FQ-PCR is sensitive,specific and stable;in naturally infected samples,the quantity of bacteria in brain was the highest;whereas in artificially infected samples,it in heart was the highest.The method developed in this study is suitable for rapid diagnosis and epidemiological investigation of RA infection.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2010年第6期756-761,共6页
Chinese Journal of Veterinary Science
基金
国家"十一五"重大科技支撑计划项目(2006BAD06A08)
