摘要
目的:研究利用微生物法测定食品中生物素含量。方法:利用植物乳杆菌(ATCC 8014)的生长与溶液中生物素含量成一定的线性关系,通过测定培养一定时间后细菌溶液吸光度的变化得出食品样品中生物素含量。结果:测得的标准曲线在0 ng～1 ng范围内线性关系良好,所测样品RSD分别为1.1%、4.1%、2.5%(n=6),平均回收率分别为84.2%、92.4%、99.7%。结论:该方法稳定可靠,适用食品中生物素含量的测定。
Objective：To develop a method of biotin determination in food by bioassay.Methods：The growth of Lactobacillus Plantarum（ATCC 8014） and the biotin content in solution are linear relationship.Biotin content in food can be measured by absorbency of bacterial solution after a certain period of time.Results：The interrelaring coefficient of the standard curve was good in the range of 0 ng～1 ng.RSD were 1.1%,4.1%,2.5%（n=6）,and the average recovery rates were 84.2%,92.4%,99.7%.Conclusion：The method can be used for biotin determination in food.
出处
《中国卫生检验杂志》
CAS
2010年第6期1406-1407,共2页
Chinese Journal of Health Laboratory Technology
