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草莓果胶裂解酶RNA干扰表达载体构建 被引量:4

Construction of RNAi Expression Vector of Pectatelyase from Strawberry
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摘要 为提高草莓果实硬度,延长货架期,利用草莓果胶裂解酶(PL)382bp的一段保守序列构建了RNA干扰植物表达载体;利用农杆菌介导将其成功转入到草莓中,通过PCR检测出2株阳性转化植株。 In order to enhance the firmness of strawberry(Fragaria ananassa Duch.)and extend the shelf life, this experiment constructed RNA interference(RNAi)expression vector using a 382 bp conservative fragment of pectate lyase. The target gene was successfully transferred into strawberry via Agrobacterium. Two postitive transformants were detected through PCR analysis.
出处 《中国蔬菜》 北大核心 2010年第12期26-30,共5页 China Vegetables
基金 重庆市科委自然科学基金项目(CSTC 2008BB1254) 中央高校基本科研业务费专项(XDJK2009C128)
关键词 草莓 果胶裂解酶 RNA干扰 遗传转化 Strawberry Pectate lyase RNAi Genetic transformation
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