摘要
目的对4日龄SD大鼠脑白质损伤(WMD)动物模型进行早期药物干预治疗,观察干预后大鼠神经行为功能改变,为早期干预治疗早产儿WMD提供依据。方法 4日龄SD大鼠58只,随机分为5组:假手术组、9 g.L-1盐水(NS)组、红细胞生成素(EPO)组、神经节苷脂(GM1)组和神经生长因子(NGF)组。假手术组新生大鼠切开颈部皮肤,分离双侧颈总动脉,再缝合颈部伤口;其余4组予以双侧颈总动脉结扎,再分别予以NS 0.01 mL.g-1、EPO 5 IU.g-1、GM1 0.02 mg.g-1、NGF 2 IU.g-1腹腔内注射,GM1组和NGF组间隔24 h重复给药1次,共5次。术后26 d,采用悬吊试验、斜坡试验和旷场试验对各组大鼠进行神经行为学检测,并测量其体质量。结果术后26 d,NS组、EPO组、GM1组和NGF组动物体质量均增长不良,神经行为能力均明显降低;NS组大鼠体质量及悬吊试验、斜坡试验和旷场试验结果与假手术组比较差异均有统计学意义;EPO组和NGF组大鼠体质量及悬吊试验、斜坡试验和旷场试验结果与NS组比较差异均有统计学意义;而GM1组悬吊试验、斜坡试验和旷场试验结果与NS组大鼠比较差异均有统计学意义。结论早期采用EPO、GM1或NGF干预治疗能够促进大鼠WMD的修复,改善其神经行为功能。
Objective To observe the neuroethology of newborn rats after early intervention in 4-day-old SD rats with white matter damage（WMD）,in order to provide evidence for early intervention in preterm infants.Methods Fifty-eight 4-day-old SD rats were randomly divided into 5 groups： sham-operated group,saline（NS） group,erythropoietin（EPO） group,ganglioside（GM1） group and nerve growth factor（NGF） group.The rats in sham-operated group were only cut open the neck skin and separated the bilateral carotid arteries without occlusion;the rats in other 4 groups were subjected to bilateral carotid artery occlusion,after they were separately intraperitoneally and injected NS 0.01 mL·g-1,EPO 5 IU·g-1,GM1 0.02 mg·g-1,NGF 2 IU·g-1,GM1 group and NGF group were repeatedly injected every 24 h,totally given 5 doses.Twenty-six days later,the rats in model groups were weighted and received neuroethology examinations,included hanging test,inclined plane test and open field test.Results Twenty-six days after operated,weight in NS group,EPO group,GM1 group and NGF group all grew slowly,and both the functions of the sense and movement and the ability of the sensibility and behavior apparently weakened in these groups.The weight and assessment of neural behavior in the NS group were significantly different compared with the sham-operated group.The weight and assessment of neural behavior in EPO group and NGF group were significantly differentce compared with those in NS group.The results of hanging test,inclined plane test and open field test in GM1 group were significantly different compared with those in NS group.Conclusions Early treatment with EPO,GM1 or NGF can advance the repair of WMD in neonatal rats,and improve the neurobehavioral functions.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2010年第12期895-897,共3页
Journal of Applied Clinical Pediatrics
