摘要
孤生受体ＣＯＵＰ－ＴＦ和ｎｕｒ７７的功能及其作用机理仍未阐明．以ＤＮＡ瞬时转染和测定氯霉素乙酰转移酶（ＣＡＴ）活性，以及凝胶阻抑测定，分析ＣＯＵＰ－ＴＦ和ｎｕｒ７７的相互作用对视黄酸应答元件（ＲＡＲＥｓ）的影响．实验表明，ＣＯＵＰ－ＴＦ通过降低ＲＡＲＥｓ的基础活性，来增强ＲＡＲＥ对视黄酸（ＲＡ）的敏感性，而ｎｕｒ７７则拮抗ＣＯＵＰ－ＴＦ的作用．ｎｕｒ７７能够加强不同ＲＡＲＥｓ的转录活性，并且与ＲＡ的诱导无关．结果证实，ｎｕｒ７７通过与ＣＯＵＰ－ＴＦ的直接作用而对ＲＡＲＥｓ产生影响。
The function of two orphan receptors,COUP TF and nur 77,and their mechanism of action remain largely unknown.The interaction between COUP TF and nur77,and their effect on retinoic acid response elements(RAREs)were investigated.In transient transfection assay and CAT assay,COUP TF increased retinoic acid(RA)sensitivity to RAREs by reducing basal activity of various RAREs.nur77 counteracted the effect of co transfected COUP TF in CV 1 cells.nur77 enhanced the transcriptional activity of various RAREs in a RA independent manner.Gel retardation assay showed that nur77 and COUP TF antagonized each other's DNA binding.These observations demonstrate that nur77 exerts its effect on RAREs through direct interaction with COUP TF,which results in inhibition of COUP TF RARE binding and transcriptional activity.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1999年第1期136-141,共6页
Chinese Journal of Biochemistry and Molecular Biology
