摘要
细胞间粘附分子－１（ＩＣＡＭ－１）是介导白细胞与内皮细胞粘附的重要粘附分子．为研究野生型ｐ５３基因对内皮细胞ＩＣＡＭ－１表达的影响，分别采用流式细胞术和ＲＴ－ＰＣＲ／ＨＰＬＣ方法测定ＩＣＡＭ－１蛋白及ｍＲＮＡ水平．静息状态的内皮细胞表面结构性地表达有少量的ＩＣＡＭ－１，在肿瘤坏死因子α（ＴＮＦα，１０～１０００Ｕ／ｍｌ）诱导下，其表达呈剂量依赖性增加．将ｐ５３基因导入内皮细胞，则显著抑制ＴＮＦα诱导的内皮细胞表面ＩＣＡＭ－１的表达．ｐ５３基因的导入对静息状态内皮细胞表面结构性表达的ＩＣＡＭ－１影响较小．ｐ５３基因主要通过降低ＩＣＡＭ－１的ｍＲＮＡ水平而抑制内皮细胞表面ＩＣＡＭ－１的表达，但对蛋白的抑制程度小于对ｍＲＮＡ的抑制程度．提示：ｐ５３基因对内皮细胞ＩＣＡＭ－１表达的影响除转录水平控制外。
Intercellular Adhesion Molecule 1(ICAM 1) is one of the most important adhesion molecules which mediate leukocyte/endothelial cell adhesion.To study the effect of wild type p53 gene on the expression of ICAM 1 in endothelial cells(EC),flow cytometry and RT PCR/HPLC were employed to evaluate the ICAM 1 protein and mRNA levels respectively.The constitutive expression of ICAM 1 was very low (10 3%±3 2%) on the surface of the resting EC.Tumor necrosis factor α(TNFα,10 1 000 U/ml) increased ICAM 1 protein expression in a dose dependent manner,with the maximum of 77 3%±2 6%.Transfection of wild type p53 gene suppressed the expression of ICAM 1 protein on the surface of EC induced by TNFα strikingly.The transfection of wild type p53 gene also affected constructive expression of ICAM 1 protein on the surface of unstimulated EC,although the suppression extent was less.Wild type p53 gene inhibited ICAM 1 protein expression in TNFα stimulated EC mainly by lowering its mRNA level.However,the reduction of ICAM 1 protein expression was less significant than that of ICAM 1 mRNA.Furthermore,wild type p53 gene slightly inhibited constructive expression of ICAM 1 protein on the surface of unstimulated EC without affecting its mRNA level.This suggests that the effect of wild type p53 gene on ICAM 1 expression is modulated not only at transcription level,but also at post transcription level.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1999年第1期6-9,共4页
Chinese Journal of Biochemistry and Molecular Biology
关键词
P53基因
基因导入
ICAM-1
内皮细胞
Wild type p53 gene,Gene transduction,Intercellular adhesion molecule 1(ICAM 1),Endothelial cell(EC)
