期刊文献+

介绍一种高效、简单的DNA沉淀法 被引量:3

在线阅读 下载PDF
导出
摘要 毫摩尔级的Zn2+可以在适宜条件下导致核酸沉淀物的形成。优化后的ZnCl2沉淀法的基本步骤是:50kb和20bp的DNA样品分别置于50mMTris(pH7.0)稀释,ED-TA到终浓度小于0.1mM中,加入0.01M磷酸钠缓冲液(pH7.0)到终浓...
作者 周政 李敏
出处 《生命的化学》 CAS CSCD 1999年第1期32-32,共1页 Chemistry of Life
  • 相关文献

同被引文献26

  • 1刘秀梅,陈艳,王晓英,计融.1992~2001年食源性疾病暴发资料分析——国家食源性疾病监测网[J].卫生研究,2004,33(6):725-727. 被引量:333
  • 2胡承香,张玉纯,李磊,顾长国.SDS-聚丙烯酰胺凝胶电泳技术的改良及其应用[J].第三军医大学学报,1996,18(1):68-70. 被引量:7
  • 3Harvey M,Brisson I,Guerin S L.A simple apparatus for fast and inexpensive recovery of DNA from polyacrylamide gels[J].Biotechniques,1993,14:942-948.
  • 4Lohmann J,Schickle H,Bosch T.REN dispaly,a rapid and efficient method for nonradioactive differential display and mRNA isolation[J].Bio Techniques,1995,18(2):200.
  • 5Sanguinetti C J,Dias N E,Simpson A J.Rapid silver staining andrecovery of PCR products separated on polyacrylamide gels[J].Biotechniques,1994,17:914-921.
  • 6Liang P, Pardee A B. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction [J]. Science, 1992, 257: 967-971.
  • 7Bachem C W B, Hoevena V A N , Ded R S, et al. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber developing[J] . Plant Journal, 1996 , 9(5): 745-754.
  • 8Opsahl F H C , Dewnff E L, Dumas C, et al. A novel endosperm-specific gene expressed in a restricted region around the maize embryo[J] . Plant Journal, 1997, 12 (1) : 235-246.
  • 9Esther V D K, Kende H. Identification of a gibberellin - induced gene in deepwater rice using differential display of mRNA[J]. Plant Mol. , 1995 , 28: 589-592.
  • 10Benito E P, Prins T, Kan J A L Application of differential display RT-PCR to the analysis of gene expression in a plant-fungus interaction[J]. Plant Mol. Biol. , 1996, 32, 947-957.

引证文献3

二级引证文献11

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部