摘要
目的研究在用微柱凝胶免疫法检测人血中头孢他啶针剂的IgM和IgG抗体时,红细胞在不同pH下被药物致敏后对检测结果的影响。方法 O型红细胞在苯巴比妥缓冲液(pH=7.0,8.0,9.6)中用头孢他啶于室温下孵育2小时后作为致敏后的红细胞,以致敏后的红细胞(pH=9.6)为初试抗原,测试600份待检血清,根据检测结果选出10份血清样品,阳性3份,弱阳性3份,阴性4份,再分别用经pH8.0及pH7.0时致敏的红细胞检测,比较结果的异同。结果红细胞在不同pH下致敏对4份阴性结果的血清和3份阳性结果的血清都没影响,但3份弱阳性标本在pH8.0时一份转阴,在pH7.0时3份都转阴。结论用微柱凝胶免疫技术检测人血中头孢他啶IgM和IgG抗体时,红细胞致敏的pH小于9.6可能出现假阴性结果。
Objective To observe the effect on gel microcolumn assay of ceftazidime IgM and/or IgG in human serum with red cells packed by drug under different pH.Methods Drug-coated red blood cells were prepared by incubating 10 volumes of ceftazidime in the barbital buffer solution（pH=7.0,8.0,9.6） for 2 hour at room temperature.600 human serum samples were prepared for the drug-coated red cells test which the incubation pH was 9.6,and 10 samples were selected according to the results.Among 10 samples three were positive,four were negative,the others were week positive All were tested by the drug-coated red cells which the incubation pH was 7.0 or 8.0.Results In the three week positive samples,one of them becomes negative when the incubation was pH8.0;all of them become negative when the incubation was pH7.0.The other seven were no different between the incubation pH was 9.6 or 8.0 or 7.0.Conclusion The results of gel microcolumn assay of ceftazidime IgM and /or IgG in Human Serum may be false negative when the incubation pH is lower than 9.6.
出处
《药学实践杂志》
CAS
2010年第3期206-208,共3页
Journal of Pharmaceutical Practice
基金
浙江省医学会临床药学科研专项(2007zyc26)
温州市科技局科研项目(Y20090193)
