摘要
目的探讨细胞凋亡与排斥反应、ＢＣＬ２及ＵＣＨＬ１表达之间的关系。方法收集１０例手术切除的无功能移植肾病理标本作为实验组，相应的移植术前活检组织作为对照组。采用免疫组织化学染色技术观测ＢＣＬ２及ＵＣＨＬ１在移植肾的表达，并观察其组织切片的形态学变化。结果对照组５０％以上的肾小管细胞ＢＣＬ２呈阳性表达，实验组ＢＣＬ２的表达明显减少（Ｐ＜０．０１），尤其是当发生急性排斥反应时；ＵＣＨＬ１的表达，对照组表达ＵＣＨＬ１的Ｔ细胞很少，而实验组的阳性表达细胞呈多灶性或成片（Ｐ＜０．０１）。凋亡细胞呈现核固缩、核破裂，或出现凋亡小体等。凋亡细胞最常见于肾小管。结论观测细胞凋亡、ＵＣＨＬ１及ＢＣＬ２的表达可作为肾移植术后监测的重要指标。
Objective To study the relationship between apoptosis and rejection, expression of BCL 2 and UCHL1.Methods The pathologic specimens of failed renal grafts from operation in 10 patients served as experimental group, and the corresponding biospy tissue before transplantation as control group. The expression of BCL 2 and UCHL1 in renal allografts was detected by using immunohistochemical staining technique in 6 cases of acute rejection, 3 cases of chronic rejection, and 1 case of cytomegalovirus infection. And the morphological changes were observed in the tissue sections. Results In control group, the expression of BCL 2 in more than 50% tubules was positive, but significantly decreased in the experimental group ( P < 0.01 ), especially in acute rejection. T lymphocytes expressing UCHL1 were significantly increased in renal allografts as compared with those in pretransplant specimens ( P < 0.01 ). Under an electron microscope, apoptosis were demonstrated.Conclusion The measurements of apoptosis, and the expression of BCL 2 and UCHL1 might play important role in the monitoring of renal allografts.
出处
《中华器官移植杂志》
CAS
CSCD
1999年第1期30-32,共3页
Chinese Journal of Organ Transplantation
