摘要
目的探讨胎脑组织移植后的存活情况及移植物与受体脑组织之间的神经纤维联系。方法以成年雄性Ｗｉｓｔａｒ大鼠为受体，胎龄为１５～１７天的Ｗｉｓｔａｒ胎鼠为供体。将胎鼠的脑皮层组织块移植至受体鼠的损毁脑腔内。术后应用环孢素Ａ预防排斥反应。术后１、３、５个月处死动物，应用免疫组织化学技术（ＡＢＣ法）进行脑组织切片染色，光镜及电镜下观察。结果同种胎脑皮层组织移植后存活率为３０％，５羟色胺能神经纤维从受体脑组织内长入移植物中，证明二者之间已建立神经纤维联系。结论胎脑移植物与受体脑组织之间可建立神经纤维联系。
Objective To investigate the survival of fetal rat cerebral cortex homografted to adult rat brain and neural connection between recipients brain and grafting tissue.Methods Normal male adult Wistar rats were used as host or recipients, and the donor tissues were obtained from 15 to 17th day fetus of Wistar rats. The donor tissues were taken from the cerebral cortex of fetus and cut into pieces which were transplanted into the brain cavity previously prepared of the recipients. Transplanted rats received daily intraperitoneal injection of 10mg/kg cyclosporin A for 2 weeks to prevent immuno rejection. All the animals were killed at different intervals(1 to 5 months)by perfusing fixative solution transcardially. The sections of rat brain specimens were stained with ABC immunohistochemistry and examined by light and electron microscope. Results Only 30% of the homografts survived and the sereotonergic like nerve fibers were projected from host brain tissue.Conclusion The grafted tissue of fetal brain cortex might survive and form neural connections with the cerebral cortex of recipients after homograft transplantation.
出处
《中华器官移植杂志》
CAS
CSCD
1999年第1期13-14,共2页
Chinese Journal of Organ Transplantation
关键词
免疫化学
神经通路
脑组织移植
胚脑
Brain/transplantation
Immunochemistry
Neural pathways
Embryo
