摘要
The diverse functions of phosphoenolpyruvate carboxylase （PEPCase; EC 4.1.1.31） in C3 plants are not as well understood as in C4 plants. To investigate the functions of PEPCase in C3 plants, rapeseed （Brassica napus L.） PEPCase gene （referred to as BNPE15） was silenced by the RNA interference （RNAi） technique. Under normal growth conditions, no significant difference in lipid content and fatty acid composition were found between wild-type （WT） and transgenic rapeseed plants. However, when these plants were subjected to osmotic stress induced by osmoticum polyethylene glycol （PEG-6000）, membrane permeability and membrane lipid peroxidization in roots and leaves of transgenic plants were higher than those of WT plants. It suggested that transgenic plants are more susceptible to osmotic stress than WT plants. Taken together, the results showed that the suppression of PEPCase by RNAi leads to susceptibility to osmotic stress in rapeseed, and PEPCase is involved in the response of C3 plants to environmental stress.
The diverse functions of phosphoenolpyruvate carboxylase （PEPCase; EC 4.1.1.31） in C3 plants are not as well understood as in C4 plants. To investigate the functions of PEPCase in C3 plants, rapeseed （Brassica napus L.） PEPCase gene （referred to as BNPE15） was silenced by the RNA interference （RNAi） technique. Under normal growth conditions, no significant difference in lipid content and fatty acid composition were found between wild-type （WT） and transgenic rapeseed plants. However, when these plants were subjected to osmotic stress induced by osmoticum polyethylene glycol （PEG-6000）, membrane permeability and membrane lipid peroxidization in roots and leaves of transgenic plants were higher than those of WT plants. It suggested that transgenic plants are more susceptible to osmotic stress than WT plants. Taken together, the results showed that the suppression of PEPCase by RNAi leads to susceptibility to osmotic stress in rapeseed, and PEPCase is involved in the response of C3 plants to environmental stress.
基金
supported by the National Natural Science Foundation of China(30770224)
