摘要
目的研究人乳头瘤病毒16型E6蛋白(human papillomavirus-16E6protein,HPV16-E6)在宫颈病变组织中的表达及其与临床病理特征之间的关系。方法采用免疫组化S-P法检测HPV16-E6蛋白在宫颈正常组织、慢性炎症、宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)、浸润癌上皮中的阳性表达情况。结果 HPV16-E6在正常宫颈、宫颈炎、CIN,宫颈癌各组中,阳性表达率分别为0.5%、38.35%、46.15%、71.11%,4组比较差异有统计学意义(χ2=27.50,P<0.01)。经χ2分割后比较(α′=0.007143),宫颈癌组、CIN组、宫颈炎组的阳性率显著高于正常宫颈组(P<0.05),宫颈癌组的阳性率显著高于宫颈炎组(P<0.01)。HPV16-E6与年龄、病理类型、宫旁或脉管浸润相关(P<0.05)。结论 HPV16-E6与宫颈癌的启动、发生、发展及侵袭转移均密切相关。HPV疫苗对于宫颈癌的防治有广泛的应用前景。
Objective To investigate the relationship between the expression of human papillomavirus-16 E6 protein （HPV16-E6） and clinical pathological characteristics. Methods The expression of HPV16-E6 protein in patients with cervical cancer, cervical intraepithelial neoplasia （CIN）, chronic cervieitis and normal cervical epithelium were examined by immunohistochemitry. Results The positive rate of HPV16-E6 protein was 71.11% ,46.15%, 38.35%, 0.5% in cervical cancer, C IN, chronic cervicitis and normal cervical epithelium respectively. There were significant differences in the positive rates among the 4 groups （ P 〈 0.01 ）. The positive rate in cervical cancer group was significantly higher than that in chronic cervicitis group and normal cervical epithelium group（ P 〈 0.01 ）. The positive rate in CIN and cervicitis group was significantly higher than that normal cervical epithelium group（ P 〈0.01 ）. The positive expression of HPV16 -E6 was correlated with the age of the patients , pathological types and the infiltration of latero - uterus or haemal tube （ P 〈 0.05 ）. Conclusion HPV16-E6 plays an important role in the genesis ,development,invasion and metastasis of cervical carcinoma. The vaccine of HPV has wide application prospect in the prevention and treatment of cervical carcinoma.
出处
《河北医药》
CAS
2010年第12期1534-1537,共4页
Hebei Medical Journal
