摘要
目的观察连接蛋白43(Cx43)在青壮年猝死综合征(SMDS)者心肌细胞的表达,并探讨其法医学意义。方法筛选法医尸检案例45例,其中SMDS组、冠心病猝死组及对照组各15例。采用免疫组织化学和图像分析技术对不同组别Cx43蛋白在心肌细胞内的表达进行阳性单位(PU)定量检测,分析Cx43蛋白在各组表达的差异。所得数据进行统计学分析。结果 SMDS组心肌Cx43染色明显减弱,阳性着色条带分布不均、深浅不一,有的呈散在颗粒状;冠心病猝死组亦见类似变化;对照组未见明显变化。经统计分析发现,3组心肌细胞内Cx43表达的PU值存在显著性差异(P<0.05)。结论 SMDS死前存在心肌缺血及心电紊乱,应属心性猝死范畴。
Objective To study the expression of connexin43 （Cx43） in cardiomyocytes in cases of sudden manhood death syndrome（SMDS） and explore its medicolegal significance. Methods Obtained 45 autopsy cases from 3 different groups （SMDS group,coronary atherosclerotic heart disease group and control group）. Cx43 protein in myocardium was detected by immunohistochemical techniques. Computerized image analysis was employed to measure the Cx43 positive units（PU） of cardiomyocytes,the data were analyzed statistically. Results Expression of Cx43 in myocardium decreased obviously in SMDS group. Similar changes were also observed in coronary atherosclerotic heart disease group but no obvious changes in the control group. There were significant differences of Cx43 positive units in the myocardium among the three groups （P0.05）. Conclusions Immunohistochemical detection of Cx43 in myocardium may effectively help postmortem diagnosis of SMDS.
出处
《中国法医学杂志》
CSCD
北大核心
2010年第3期159-161,共3页
Chinese Journal of Forensic Medicine
基金
国家自然科学基金资助项目(39700167)
关键词
法医病理学
连接蛋白43
青壮年猝死综合征
免疫组织化学
forensic pathology
connexin43（Cx43）
sudden manhood death syndrome（SMDS）
immunohistochemical method
