摘要
目的 探讨纤丝状Aβ^(42)对GSK-3β和PP2A Cα表达的影响。方法 应用立体定向技术对15月龄雄性SD大鼠进行海马内注射纤丝状Aβ^(42)或双蒸水,采用免疫组织化学染色方法,观察海马神经元GSK-3β和PP2A Cα的表达改变,并进行图像分析。结果 AB_(42)组海马神经元GSK-3β的表达明显高于双蒸水组,但两组的海马神经元PP2A Cα的表达没有明显差异。结论 纤丝状Aβ_(42)能使海马神经元GSK-3β表达上调,而对PP2A Cα的表达没有明显影响,提示纤丝状Aβ_(42)诱导tau蛋白过度磷酸化可能与GSK-3β表达上调有关。
Objective To explore the effects of fibrillar amyloid-beta peptide-42 (Aβ_(42)) on expressions of glycogen synthase kinase-3 (GSK-3β) and phosphatase 2A (PP2A) Ca. Methods Stereotaxic intrahippocampal microinjections of fibrillar Aβ_(42)or double-distilled water (ddH20) into 15-month SD male rats were performed and the expressions of GSK-3β and PP2A Cα in hippocampal neurons were observed immunohistochemically. Results The expressions of GSK-3β in the hippocampal neurons of fibrillar Aβ_(42) group were significantly stronger than those of ddH_2O group. However, the expressions of PP2A Cα in hippocampal neurons of both groups were of no significant difference. Conclusion Fibrillar Aβ_(42) induced higher expression of GSK-3β but had no significant effect on the expression of PP2A Cα in hippocampal neurons, It suggests that hyperphosphorylation of tau protein induced by fibril lar Aβ_(42) may contribute to the higher expression of GSK-3β.
出处
《中华神经医学杂志》
CAS
CSCD
2004年第4期248-250,共3页
Chinese Journal of Neuromedicine
基金
广州市科委科技项目基金(JB02 2000-2-026-01)
