摘要
探讨组织金属蛋白酶类在人胃癌组织中的表达。方法：应用免疫组织化学（ＳＰ法）和原位杂交（ｃＤＮＡ－ｍＲＮＡ）技术对４１例入胃癌和１７例癌旁组织中的金属蛋白酶ＭＭＰ－２、ＭＴ－ＭＭＰ基因进行检测。结果：（１）免疫组化分析ＭＭＰ－２、ＭＴ－ＭＭＰ蛋白在胃癌组织的阳性率分别为７３．１％（３０／４１）和６８．３％（２８／４１），在癌旁组织的阳性率分别是２２．５％（４／１７）和４１．１％（７／１７）。（２）原位杂交分析胃癌组织ＭＭＰ－２及ＭＴ－ＭＭＰｍＲＮＡ阳性分别为７０．７％（２９／４１）和５６．０％（２３／４１），癌旁组织分别为２３．５％（４／１７）和３５．２％（６／１７）。一致性检验显示二种基因的免疫组化和原位杂交检测结果的关系密切（ｒ＝０．９９８ ４，Ｐ＜０．０５）。结论：提示ＭＭＰｓ基因的过度表达参与了胃癌的发生发展过程，同时证实ＭＭＰｓ的免疫组化是检测胃癌组织侵袭、转移倾向的可靠方法，为建立肿瘤转移的分子标志提供了理论和实验依据。
To evaluate the relationship of over-expression of metalloproteinases (MMP-2 and MMP-MT) and gastric carcinoma. Methods: Immunohistochemistry (SP-A methods) and in situ in hybrization (cDNA-mRNA) were used to de detect MMP-2, MAP-9 and W-MMP gene expression in human gastric carcinoma(HGC)and nearby non-tumorous tissues. Results:The positive rates of MMP-2 and MT-MMP were 73. 1 % (30/41),68. 3 % (28/41) and 22. 5% (4/17),41.1% (7/ 17) in HGC and in NT respectively. The positive rates of MMP-2 and MT-MMP mRNA were 70. 7% (29/41 ), 56. 0% (23/41) and 23. 5% (4/17), 35 .2% (6/17) in HGC and NT respectively. The expression of MMP-2 and MT-MMP at protein level was significantly correlated with that at mRNA level. Conclusions: There was a close connection of MMP-2 and MT-MMP gene over-expression with gastrocarcinogenesis. Immunohistochemistry (SP method) on section of paraffin embedded tissue is a reliable method for detection of MMP-2 and MT-MMP gene expression in HGC.
出处
《天津医药》
CAS
1999年第1期3-5,共3页
Tianjin Medical Journal
基金
全军95重点攻关课题资助
关键词
胃肿瘤
免疫组织化学
原位杂交
金属蛋白酶类
gastric carcinoma immunohistochemistry in situ hybrization metalloproteinases
