摘要
背景:毛囊干细胞在受到外界环境刺激作用下,例如创伤,化学伤等,其理化性质及细胞状态会有所变化。但是从目前文献来看,涉及其在皮肤烫伤中变化的研究的比较少。目的:初步观察大鼠烫伤模型中,毛囊隆突区β神经生长因子的表达变化以及对烫伤组织愈合的影响。方法:随机选取30只乳鼠,采用90℃水温持续3s时间的方式制作大鼠触须部表皮组织浅Ⅱ度烫伤模型,造模后第12,24,36,48,60小时对毛囊隆突区进行分离,并进行匀浆,以同窝新生6只乳鼠正常唇部组织作为对照。采用Brandford法进行总蛋白检测及ELISA定量检测β神经生长因子。免疫组织化学进行神经生长因子的定性半定量检测。结果与结论:在烫伤后各时间点检测的β神经生长因子与总蛋白比值中,β神经生长因子的表达在烫伤后36h开始后增高,60h达到峰值。组织切片中同样发现这一规律。通过图像软件分析表明,提取β神经生长因子蛋白检测与组织水平β神经生长因子表达一致。提示烫伤后12～60h时间段内,烫伤后60h隆突区表达的β神经生长因子达到峰值,对烫伤愈合的修复作用也达到峰值。隆突区是毛囊干细胞的巢,此部位β神经生长因子表达增强可能对毛囊干细胞的分化和组织修复具有一定的积极意义。
BACKGROUND： The surroundings stimulation, such as wound and chemical injury, will result in changes of hair follicle stem cells （HFSC）. Up to date, few articles indicate the effect of burn on HFSCs. OBJECTIVE： To investigate the changes of β-nerve growth factor （β-NGF） level in vibrissa follicle bulge region and its effect on the healing of neonatal rats. METHODS： A total of 30 neonatal rats were selected to prepare burn models by giving 90 ℃ boiled tap water for 3 seconds, and vibrissa follicle bulge was separated at 12, 24, 36, 48 and 60 hours postoperatively. The remained 6 neonatal rats were served as controls. Total protein concentration was measured by Brandford method. The expression of β-NGF was detected by ELISA. Meanwhile, the tissues in each time points were collected for frozen section and immunohistochemistry. RESULTS AND CONCLUSION： Among the tested total protein, the expression of the β-NGF increased after 36 hours, reached a peak at 60 hours after burn. The identical changes were found in tissue slices. The image analysis demonstrated that the β-NGF protein detection was coincident with β-NGF expression in tissues. During 12 hours to 60 hours after burn, the expression of the β-NGF in the bulge area peaked at 60 hours; simultaneously, the repairing effect of healing also reached a peak. Bulge area is a nest of HFSC, the enhanced expression of β-NGF may have some positive significance to the differentiation of the HFSC and tissue repair.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第11期1987-1991,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
四川省教育厅青年基金资助项目(07ZB016)~~
