摘要
本文根据抗原分子特性，用限制性内切酶ＨｐａⅠ和ＥｃｏＲⅠ及三对ＸｈｏⅠ－ＥｃｏＲⅠ接头，对β－半乳糖苷酶融合蛋白表达载体ｐＷＲ４５０进行了改建，截去其β－半乳糖苷酶Ｃ端约３００个氨基酸的编码序列，构建了一组含β－半乳糖苷酶Ｎ端１４６个氨基酸编码序列的ｐＷＸ１４６融合蛋白表达载体，以ｐＷＸ１４６－Ⅰ作为载体。
We have constructed a pWX146 family based on pWR450,a β galactosidase fusion protein expression vector.The pWX146 family is consisted of pWX146,pWX146 1 and pWX146 2.Each of them includes a lac promoter of E.coli,a truncated sequence encoding 146 amino acids at the N terminus of β galactosidase and a different poly linker sequence.A 306bp gene fragment,encoding repetitive region of histidine rich protein Ⅱ(HRP Ⅱ) of Plasmodium,falcipatum was cloned into pWX146 1 and efficiently expressed fusion protein after induction with IPTG in E.coli.The truncation of the sequence encoding β galactosidase minimized influence on the stucture and expression of the expected target peptides,therefore the pWX146 family may also be useful for epitope analysis using sequential deletion strategy.
出处
《寄生虫与医学昆虫学报》
CAS
1999年第1期12-17,共6页
Acta Parasitologica et Medica Entomologica Sinica
基金
上海市青年科技启明星计划资助
关键词
融合蛋白
表达载体
疟原虫
Fused protein \ Expression vector Reconstruction
