摘要
目的:应用基于核磁共振氢谱(nuclear magnetic resonance1H,1H-NMR)的代谢组学方法比较慢性乙肝和重症乙肝患者血清代谢组。方法:对慢性乙肝患者血清(20例)和重症乙肝患者血清(11例)进行核磁共振氢谱检测,应用主成分分析方法找出慢性乙肝和重症乙肝患者血清代谢组差异。结果:与慢性乙肝患者血清代谢组比较,重肝患者血清中蛋氨酸、乙酰乙酸盐、三甲胺、葡萄糖和乳酸升高,而胆碱和脂的含量降低。结论:慢性乙肝和重症乙肝患者血清代谢组具有明显差异,基于核磁共振氢谱和主成分分析的代谢组学方法可以为重症乙肝的诊断提供代谢依据。
Objective：To compare the metabolites of serum from chronic and severe HBV patients by using nuclear magnetic resonance ^1H（^1H-NMR） spectroscopy.Methods：1H-NMR spectra were measured for serum samples from 20 chronic HBV patients and 11 severe HBV patients,and the spectra data were analyzed by principal component analysis（PCA） to reveal the metabonomic changes.Results：Obvious metabonomic differences existed between chronic HBV patients and severe HBV patients which were highlighted by the increased levels in methionine,acetoacetate,TMA,glucose and lactate together with the decreased levels of choline and lipids in severe HBV patients.Conclusion：The metabonomes of serum from severe HBV patients differ markedly from those of the chronic HBV patients,which indicates that serum 1H-NMR sepctroscopy in conjunction with PCA offers an efficient tool for understanding the biochemistry of severe hepatitis B.
出处
《中国医药导报》
CAS
2010年第18期27-28,30,共3页
China Medical Herald
基金
广东药学院人才建设专项基金
博士启动基金(2007JCX01)
关键词
重症乙肝
代谢
核磁共振氢谱
主成分分析
Severe hepatitis B
Metabonomics
Nuclear magnetic resonance
Principal component analysis
