摘要
四(4-三甲胺苯)卟啉与单抗体3B6结合后,有显著的增色效应,反映了卟啉与抗体是刚性紧密结合的,抗体的抗原结合部位有芳香族氨基酸存在,并且产生巨大的诱导效应.用紫外(UV)可见和圆二色谱(CD)分析四(4-三甲胺苯)卟啉同抗体的结合,表明(四(4-三甲胺苯)卟啉与单抗体,既有1∶1结合又有2∶1结合.
The combination between tetra(tera-trimethy lamine benzene) prophyrin and McAb 3B6 resulted in a significantly hyperchromic effect,which indicated rigidity and compactness of the combination,and existence of the aromatic amino acids at the combination sites of antigen in antibody.In the nean time,the inducement to McAb 3B6 from the porphyrin resulted in a large induced Cotton effect(ICD). Quantitative analyses of the combination between porhpyrin and antibody by ICD showed that not only 1∶1 combination occurred in excess of antibody over porphyrin.
出处
《分子科学学报》
CAS
CSCD
2004年第3期60-62,共3页
Journal of Molecular Science
基金
国家自然科学基金资助项目(2971005)
中国科学院基础局重大资助项目(KJ951-AI-504-02)
国家杰出青年科学基金资助项目(29625203)
