摘要
为寻找克服Ｐ糖蛋白（Ｐｇｐ）介导肿瘤多药耐药的途径，选择环孢霉素Ａ（ＣｓＡ）作为耐药逆转剂，分别采用台盼蓝拒染法、ＭＴＴ法和免疫组化法分析ＣｓＡ对人白血病细胞株的毒性及逆转效果，观察ＣｓＡ及长春新碱（ＶＣＲ）对Ｋ５６２／ＶＣＲ生长的抑制作用及检测Ｋ５６２／ＶＣＲ细胞膜Ｐｇｐ的表达情况。结果显示，ＣｓＡ在非毒性剂量浓度４μｇ／ｍｌ以下能逆转细胞耐药，与ＶＣＲ合用能抑制Ｋ５６２／ＶＣＲ生长（Ｐ＜０．０１）；Ｋ５６２／ＶＣＲ细胞膜上Ｐｇｐ的高表达，经ＣｓＡ作用后其表达下降。这表明ＣｓＡ能逆转Ｋ５６２／ＶＣＲ耐药性。
To explore approaches to overcoming P glycoprotein(P gp) mediated multidrug resistance(MDR) of tumor cells,the reversal effects of Cyclosporin A(CsA) on multidrug resistance of human leukemic cell line K562/VCR were studied with Taipanlan dye exclusion,MTT colorimetric and immunochemical assay.The inhibition of K562/VCR growth and P gp expression of K562/VCR cellular membrance were examined.The results showed that CsA reverse the drug resistance of K562/VCR cells,which is related to P gp expression.
出处
《实用癌症杂志》
1999年第1期9-10,共2页
The Practical Journal of Cancer
